Pinoxaden is the one of the acetyl-CoA carboxylases (ACCase) inhibiting herbicides and used for controlling grass weeds. In this study, cyto-genotoxic effects of Pinoxaden on the Allium cepa roots were investigated using Allium ana-telophase and comet assays by determining the root growth, mitotic index (MI), mitotic phases, chromosomal aberrations (CAs) and DNA damage. Different concentrations of Pinoxaden from 0.5 to 100 mg/L were employed on root tips for 96 h to find the effective concentration that reduces root tip elongation by 50% in comparison with negative control (EC₅₀). Pinoxaden concentrations of 1.25 mg/L (1/2xEC₅₀), 2.5 mg/L (EC₅₀) and 5 mg/L (2xEC₅₀); methyl methane sulphonate (MMS, 10 mg/L) for positive control and distilled water for negative control were exposed to Allium bulbs for several time intervals (24, 48, 72 and 96 h). Pinoxaden showed cytotoxic effects by decreasing the root growth and MI. Pinoxaden induced CAs including disturbed ana-telophase, anaphase bridges, chromosome laggards, stickiness, polyploidy, micronucleus at 5 mg/L, c-metaphase and binuclear cells and also DNA damage compared with control group. The current study confirmed cyto-genotoxic effects of Pinoxaden. Further research is needed to clarify the cyto-genotoxic mechanisms of Pinoxaden at molecular level.

中文翻译：

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Pinoxaden 对洋葱根的细胞遗传毒性作用。

Pinoxaden 是一种乙酰辅酶 A 羧化酶 (ACCase) 抑制性除草剂，用于控制禾本科杂草。在这项研究中，通过测定根生长、有丝分裂指数 (MI)、有丝分裂期、染色体畸变 (CA) 和 DNA 损伤，使用葱末末期和彗星试验研究了匹诺沙登对洋葱根的细胞遗传毒性作用。将 0.5 至 100 mg/L 的不同浓度的 Pinoxaden 用于根尖 96 小时，以找到与阴性对照 (EC ₅₀ )相比将根尖伸长减少 50% 的有效浓度。品诺沙登浓度为 1.25 mg/L (1/2xEC ₅₀ )、2.5 mg/L (EC ₅₀ ) 和 5 mg/L (2xEC ₅₀); 用于阳性对照的甲基磺酸甲酯 (MMS, 10 mg/L) 和用于阴性对照的蒸馏水在几个时间间隔（24、48、72 和 96 小时）暴露于葱属球茎。Pinoxaden 通过降低根生长和 MI 显示出细胞毒性作用。与对照组相比，品诺沙登诱导的 CA 包括扰乱的后期、后期桥、染色体滞后、粘性、多倍体、5mg/L 的微核、c-中期和双核细胞以及 DNA 损伤。目前的研究证实了匹诺沙登的细胞遗传毒性作用。需要进一步研究以在分子水平上阐明 Pinoxaden 的细胞遗传毒性机制。