The dietary calcium requirement of fingerling Labeo rohita (6.0 ± 0.11 cm; 1.94 ± 0.07 g) was examined using seven iso-nitrogenous and iso-caloric purified diets (350 g kg⁻¹ crude protein; 16.72 kJ g⁻¹ gross energy) with incremental levels of calcium (2.14, 3.12, 4.23, 5.11, 6.14, 8.23, and 11.24 g kg⁻¹) that were hand-fed to triplicate groups of fish (20 fish per tank) to apparent satiation for 8 weeks. At the end of the feeding trial, ten fish from each replicate tank (n = 10 × 3) were sampled to obtain serum, scales, and vertebrae. The remaining fish in each tank were sampled to determine proximate chemical body composition. Live weight gain (LWG; 637.6%), protein gain (PG; 2.19 g fish⁻¹), protein retention efficiency (29.89%), and feed conversion ratio (1.69) improved with increased dietary calcium up to 5.11 g kg⁻¹ and then stabilized. Dietary calcium up to 5.11 g kg⁻¹ significantly increased calcium and phosphorus concentrations of whole body, vertebrae, and scales. Fish fed a diet with calcium higher than 5.11 g kg⁻¹ had a significant decrease (P < 0.05) in magnesium and zinc, while potassium was unaffected. Serum alkaline phosphatase activity increased (P < 0.05) with increased dietary calcium up to 5.11 g kg⁻¹, but serum calcium and phosphorus concentrations remained unchanged. Based on broken-line regression analysis of LWG, PG, whole body, vertebrae, and scale calcium content against dietary calcium concentration, dietary calcium in the range of 5.16–5.48 g kg⁻¹ is recommended for fingerling L. rohita.

使用七种等氮和等热量的纯饮食（350 g kg ^-1粗蛋白； 16.72 kJ g ^-1总能量）检测了鱼种Labeo rohita（6.0±0.11 cm; 1.94±0.07 g）的饮食钙需求人工饲喂三组鱼（每罐20条鱼）的钙（2.14、3.12、4.23、5.11、6.14、8.23和11.24 g kg ^-1）的增量水平持续8周。在饲养试验结束时，从每个重复缸中取10条鱼（n  = 10×3），以获取血清，鱼鳞和椎骨。采样每个水箱中剩余的鱼，以测定其化学成分。活体重增加（LWG; 637.6％），蛋白质增加（PG; 2.19 g鱼^-1），随着日粮钙含量增加至5.11 g kg ^-1之后，蛋白质保留效率（29.89％）和饲料转化率（1.69）得到改善，然后稳定下来。膳食钙含量最高为5.11 g kg ^-1时，会显着增加全身，椎骨和鳞片的钙和磷浓度。饲粮中钙含量高于5.11 g kg ^-1的鱼的 镁和锌含量显着下降（P <0.05），而钾盐未受影响。 饮食中钙含量增加至5.11 g kg ^-1时，血清碱性磷酸酶活性增加（P <0.05），但血清钙和磷浓度保持不变。基于LWG，PG，全身，椎骨和鳞片钙含量相对于饮食钙浓度的折线回归分析，建议将鱼种的L. rohita的饮食钙含量控制在5.16–5.48 g kg ^-1之间。