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A method to analyze the relationship between locomotor activity and feeding behaviour in larvae of Betta splendens
Aquaculture International ( IF 2.9 ) Pub Date : 2020-02-14 , DOI: 10.1007/s10499-020-00516-1
José Gilmar da Silva Souza , Lais Teodoro Libeck , Bárbara do Carmo Rodrigues Virote , Renata Catão Egger , Gabriel Cândido Ribeiro de Sá , Gilmara Junqueira Machado , Luis David Solis Murgas

The transition from live to artificial feed is a critical step for fish larvae with potential for cultivation. Determining the moment to initiate the introduction of artificial feed based on larvae swimming behaviour can reduce mortality levels resulting from this dietary transition. Kinovea® is a cost-free software used as a tool to study sports kinematics; its use can help to establish locomotor parameters to determine the best time to introduce artificial feed in Siamese fighting fish larvae diets, reducing the negative effects of weaning. The objective in this study was to evaluate the locomotor behaviour of Siamese fighting fish larvae under different feeding regimes using Kinovea® as a tool to study fish locomotor behaviour. A total of 234 larvae of Siamese fighting fish (Betta splendens) were used; they were divided into three dietary strategies (treatments): live feed, artificial feed and co-feeding. Each treatment had four replicates. The experimental units were video recorded for 1 min after feed was offered. It was observed that the weaning strategy resulted in higher zootechnical performance (p < 0.05). Larvae fed exclusively with live feed had higher swimming speed, and larvae fed with artificial feed displayed low velocity (p < 0.05). The results indicate that the use of artemia nauplii and high swimming speed are correlated. Based on the results, it is possible to conclude that the weaning strategy proposed in this study is efficient and that Kinovea® can be used as a software to access locomotor behaviour for fish larvae.



中文翻译:

Betta splendens幼虫自发活动与摄食行为关系的分析方法。

从有生命的饲料向人工饲料的过渡是具有发展潜力的鱼幼虫的关键步骤。确定根据幼体游动行为开始引入人工饲料的时机可以降低这种饮食过渡带来的死亡率。Kinovea®是一种免费软件,可用于研究运动学;它的使用可以帮助确定运动参数,以确定在暹罗斗鱼幼虫日粮中引入人工饲料的最佳时间,从而减少了断奶的负面影响。本研究的目的是使用Kinovea®作为研究鱼类运动行为的工具,评估暹罗斗鱼幼虫在不同喂养方式下的运动行为。共有234条暹罗斗鱼Betta splendens)被使用;它们分为三种饮食策略(治疗):活饲料,人工饲料和共同喂养。每种处理均重复四次。提供饲料后,对实验单位进行视频记录1分钟。观察到断奶策略导致更高的动物技术性能(p  <0.05)。仅用活饲料喂养的幼虫游动速度较高,而用人工饲料喂养的幼虫的游动速度较低(p  <0.05)。结果表明,使用无节律性无节幼体和高游泳速度是相关的。根据结果​​,可以得出结论,本研究中提出的断奶策略是有效的,并且Kinovea®可以用作访问鱼幼虫运动行为的软件。

更新日期:2020-02-14
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