BACKGROUND Cutaneous leishmaniasis (CL) is well linked with immunogenetic factors. This study was undertaken to test the association of TNF-α - 308 and IFN-γ + 874 gene polymorphisms with the susceptibility of Leishmania (L) species among CL patients in central region of Saudi Arabia. METHODS This is a case-control study involved 169 Saudi subjects with different L. species and 199 healthy controls from central region of Saudi Arabia. All subjects were characterized by TNF-α - 308 G/A and IFN-γ + 874 A/T gene polymorphisms using PCR. RESULTS Evaluation of genotyping and allelic frequency of TNF-α - 308 G/A in different L. species showed no significant association compared to controls (p > 0.05). Except, in cases of L. tropica that showed significantly higher TNF-α - 308 A versus G allele frequency (p = 0.0004). Evaluation of genotyping of IFN-γ + 874 (TT versus AA+AT recessive) and allelic frequency of IFN-γ + 874 (T versus A) showed significant higher in L. major and also in total CL cases as compared to healthy controls (p < 0.05). Furthermore, a strong association was observed between the susceptibility of L. major, L. tropica or total CL cases with synergistically combined high TNF-α 308/INF-γ 874 alleles. CONCLUSIONS This is the first report that shows the gene polymorphisms of TNF-α - 308 G/A and IFN-γ + 874 A/T in Saudi patients with different L. species infections. Data showed that the TNF-α-308 G/A gene polymorphism is not associated with the susceptibility of CL in Saudi subjects. The only correlation was found in between A versus G allelic frequency in L. tropica. Importantly, IFN-γ + 874 A/T polymorphism was found to be associated with the susceptibility of L. major and also with total CL subjects. Moreover, data from synergistically combined high TNF-α 308/INF-γ 874 alleles strongly suggest their potential role in the susceptibility of leishmania infection.

中文翻译：

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沙特阿拉伯皮肤利什曼病患者的TNF-α-308 G / A和IFN-γ+ 874 A / T基因多态性。

背景技术皮肤利什曼病（CL）与免疫遗传因子紧密相关。这项研究旨在测试沙特阿拉伯中部地区CL患者中TNF-α-308和IFN-γ+ 874基因多态性与利什曼原虫（L）菌种的易感性之间的关系。方法这是一个病例对照研究，涉及来自沙特阿拉伯中部地区的169名不同L.物种的沙特受试者和199名健康对照。使用PCR，所有受试者的特征在于TNF-α-308 G / A和IFN-γ+ 874 A / T基因多态性。结果在不同的L.物种中，TNF-α-308 G / A的基因分型和等位基因频率评估与对照组相比无显着相关性（p> 0.05）。除外，在L. tropica中，TNF-α-308 A比G等位基因频率高得多（p = 0.0004）。与健康对照组相比，大肠念珠菌和总CL病例中IFN-γ+ 874的基因分型评估（TT与AA + AT隐性比较）和IFN-γ+ 874的等位基因频率显着更高（ p ＜0.05）。此外，在大乳酸杆菌，热带乳酸杆菌或总CL病例与协同结合的高TNF-α308 /INF-γ874等位基因的易感性之间观察到强烈关联。结论这是第一份显示沙特氏菌不同感染沙门氏菌患者TNF-α-308 G / A和IFN-γ+ 874 A / T基因多态性的报告。数据显示，TNF-α-308G / A基因多态性与沙特阿拉伯受试者的CL易感性无关。发现唯一的相关性是在热带乳杆菌的A与G等位基因频率之间。重要的，发现IFN-γ+ 874 A / T多态性与大乳酸杆菌的敏感性以及总的CL受试者有关。此外，来自协同结合的高TNF-α308 /INF-γ874等位基因的数据强烈表明其在利什曼原虫感染易感性中的潜在作用。