Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
A paper-based conductive immunosensor for the determination of Salmonella Typhimurium.
Analyst ( IF 3.6 ) Pub Date : 2020-05-13 , DOI: 10.1039/d0an00515k Wanida Wonsawat 1 , Sunisa Limvongjaroen , Sathaporn Supromma , Wattana Panphut , Nipapan Ruecha , Nalin Ratnarathorn , Wijitar Dungchai
Analyst ( IF 3.6 ) Pub Date : 2020-05-13 , DOI: 10.1039/d0an00515k Wanida Wonsawat 1 , Sunisa Limvongjaroen , Sathaporn Supromma , Wattana Panphut , Nipapan Ruecha , Nalin Ratnarathorn , Wijitar Dungchai
Affiliation
|
We report for the first time a highly sensitive and rapid quantitative method for the detection of Salmonella Typhimurium (S. Typhimurium) using a conductive immunosensor on a paper-based device (PAD). S. Typhimurium monoclonal antibodies (MA) were first immobilized on a paper-based device and then captured by S. Typhimurium. After an immunoreaction on the device, the polyclonal antibody-colloidal gold conjugate (PA-AuNPs) was dropped to bind with S. Typhimurium. After a complete sandwich reaction, a dark red color appeared on the paper-based device, which can be observed by the naked eye for a rapid screening test. The electrical conductivity of PA-AuNPs between the screen-printed electrodes on the paper-based device was also measured for an accurate quantitative analysis. The electrical conductivity correlated well with the concentration of S. Typhimurium, which was controlled by the amount of S. Typhimurium attached to the polyclonal antibody-colloidal gold conjugate. The device showed a linear correlation for the concentration of the S. Typhimurium in the range of 10–108 CFU mL−1 in a logarithmic plot, with an R2 value of 0.9882 and a limit of detection (LOD) as low as 10 CFU mL−1. This simple, highly sensitive, and rapid method for the S. Typhimurium detection was successfully performed within 30 min, and it can be developed into small portable measuring devices in order to facilitate preliminary screening tests.
中文翻译：
纸基导电免疫传感器，用于确定鼠伤寒沙门氏菌。
我们首次报告了一种高灵敏度，快速的定量方法，用于在纸质设备（PAD）上使用导电免疫传感器检测鼠伤寒沙门氏菌（S. Typhimurium）。鼠伤寒沙门氏菌单克隆抗体（MA）首先固定在基于纸张的设备上，然后由鼠伤寒沙门氏菌捕获。在设备上进行免疫反应后，滴下多克隆抗体-胶体金结合物（PA-AuNPs）以与鼠伤寒沙门氏菌结合。三明治反应完成后，基于纸的设备上会出现深红色，可以用肉眼观察到该颜色，以进行快速筛选测试。还对纸基设备上丝网印刷电极之间的PA-AuNPs的电导率进行了测量，以进行准确的定量分析。电导率与鼠伤寒沙门氏菌的浓度密切相关，鼠伤寒沙门氏菌的浓度由附着于多克隆抗体-胶体金偶联物上的鼠伤寒沙门氏菌的量控制。在对数图中，该设备显示鼠伤寒沙门氏菌浓度在10–10 8 CFU mL -1范围内具有线性关系，R 2值为0.9882，检测限（LOD）低至10 CFU mL -1。这种简单，高度灵敏，快速的鼠伤寒沙门氏菌检测方法已在30分钟内成功完成，可以将其开发成小型便携式测量设备，以利于初步筛选测试。
更新日期：2020-06-29
中文翻译：
纸基导电免疫传感器，用于确定鼠伤寒沙门氏菌。
我们首次报告了一种高灵敏度，快速的定量方法，用于在纸质设备（PAD）上使用导电免疫传感器检测鼠伤寒沙门氏菌（S. Typhimurium）。鼠伤寒沙门氏菌单克隆抗体（MA）首先固定在基于纸张的设备上，然后由鼠伤寒沙门氏菌捕获。在设备上进行免疫反应后，滴下多克隆抗体-胶体金结合物（PA-AuNPs）以与鼠伤寒沙门氏菌结合。三明治反应完成后，基于纸的设备上会出现深红色，可以用肉眼观察到该颜色，以进行快速筛选测试。还对纸基设备上丝网印刷电极之间的PA-AuNPs的电导率进行了测量，以进行准确的定量分析。电导率与鼠伤寒沙门氏菌的浓度密切相关，鼠伤寒沙门氏菌的浓度由附着于多克隆抗体-胶体金偶联物上的鼠伤寒沙门氏菌的量控制。在对数图中，该设备显示鼠伤寒沙门氏菌浓度在10–10 8 CFU mL -1范围内具有线性关系，R 2值为0.9882，检测限（LOD）低至10 CFU mL -1。这种简单，高度灵敏，快速的鼠伤寒沙门氏菌检测方法已在30分钟内成功完成，可以将其开发成小型便携式测量设备，以利于初步筛选测试。
京公网安备 11010802027423号