The publication of reproducible, replicable, and translatable data in studies utilizing animal models is a scientific, practical, and ethical necessity. This requires careful planning and execution of experiments and accurate reporting of results. Recognition that numerous developmental, environmental, and test-related factors can affect experimental outcomes is essential for a quality study design. Factors commonly considered when designing studies utilizing aquatic animal species include strain, sex, or age of the animal; water quality; temperature; and acoustic and light conditions. However, in the aquatic environment, it is equally important to consider normal species behavior, group dynamics, stocking density, and environmental complexity, including tank design and structural enrichment. Here, we will outline normal species and social behavior of 2 commonly used aquatic species: zebrafish (Danio rerio) and Xenopus (X. laevis and X. tropicalis). We also provide examples as to how these behaviors and the complexity of the tank environment can influence research results and provide general recommendations to assist with improvement of reproducibility and replicability, particularly as it pertains to behavior and environmental complexity, when utilizing these popular aquatic models.

中文翻译：

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行为、社会和环境因素对水生动物模型的再现性和可复制性的影响。


在利用动物模型的研究中发表可重复、可复制和可翻译的数据是科学、实用和伦理的必要性。这需要仔细规划和执行实验以及准确报告结果。认识到许多发育、环境和测试相关因素会影响实验结果对于高质量的研究设计至关重要。设计利用水生动物物种的研究时通常考虑的因素包括动物的品系、性别或年龄；水质；温度;以及声学和光条件。然而，在水生环境中，考虑正常物种行为、群体动态、饲养密度和环境复杂性（包括水箱设计和结构富集）也同样重要。在这里，我们将概述 2 种常用水生物种的正常物种和社会行为：斑马鱼 ( Danio rerio ) 和非洲爪蟾( X. laevis 和 X.tropicalis ) 我们还提供了一些示例，说明这些行为和水族箱环境的复杂性如何影响影响研究结果并提供一般建议，以帮助提高可重复性和可复制性，特别是在使用这些流行的水生模型时涉及行为和环境复杂性。