Recent research has demonstrated that chemerin may take part in the regulation of reproduction. The aim of this study was to determine the expression of chemerin system – chemerin and its receptors, chemokine-like receptor 1 (CMKLR1), G protein-coupled receptor 1 (GPR1) and C-C chemokine receptor-like 2 (CCRL2) – in the porcine uterus during the oestrous cycle and early pregnancy, and in trophoblasts and conceptuses by real-time PCR and western blotting. Chemerin concentrations in uterine luminal flushings (ULF) were determined using ELISA test. In the endometrium, the highest expression of chemerin and GPR1 proteins was observed during the mid-luteal phase; CMKLR1, during the late luteal phase; and CCRL2, during the follicular phase of the cycle. In the myometrium, chemerin protein expression was enhanced during the early luteal phase, and chemerin receptor proteins were highly expressed during the follicular phase. In the endometrium of pregnant pigs, the highest expression of chemerin and CCRL2 protein was observed during implantation; CMKLR1, during placentation; and GPR1, during embryo migration. In the myometrium, chemerin and CCRL2 protein expression increased at the end of implantation, and the expression of CMKLR1 and GPR1 protein was enhanced during implantation. In the conceptuses and trophoblasts, the highest expression of chemerin system proteins was observed during placentation, with the exception of GPR1 protein in the trophoblasts. The highest concentrations of the analysed adipokine were observed in ULF during the luteal phase of the cycle and during maternal recognition of pregnancy. This is the first study to demonstrate that the expression of the chemerin system in the porcine uterus, conceptuses and trophoblasts, and chemerin concentrations in ULF are influenced by the hormonal milieu in different stages of the oestrous cycle and in early pregnancy. The present results also suggest that chemerin is implicated in the regulation of reproductive functions in pigs.

最近的研究表明凯莫瑞可能参与生殖的调节。这项研究的目的是确定chemerin系统（chemerin及其受体，趋化因子样受体1（CMKLR1），G蛋白偶联受体1（GPR1）和CC趋化因子受体样2（CCRL2）的表达。通过实时PCR和Western印迹检测在雌性周期和早孕期间的猪子宫，以及在滋养细胞和受孕中的猪子宫。子宫腔冲洗液中的chemerin浓度（ULF）是使用ELISA试验确定的。在子宫内膜中，黄体中期观察到了凯莫瑞和GPR1蛋白的最高表达。在黄体后期，CMKLR1；和CCRL2，在周期的卵泡期。在子宫肌层中，在黄体早期阶段，chemerin蛋白的表达增强，而在卵泡期，chemerin受体的蛋白高度表达。在妊娠猪的子宫内膜中，在植入过程中观察到了凯莫瑞和CCRL2蛋白的最高表达。CMKLR1，在分娩过程中；和GPR1，在胚胎移植过程中。在子宫肌层，chemerin和CCRL2蛋白的表达在植入结束时增加，而CMKLR1和GPR1蛋白的表达在植入过程中增强。在概念和滋养细胞中 除胎盘滋养层中的GPR1蛋白外，胎盘素中chemerin系统蛋白的表达最高。在周期的黄体期和孕产妇识别期间，在ULF中观察到最高的分析型脂肪因子浓度。这是第一项证明凯莫瑞系统在猪子宫，概念性子宫和滋养细胞中的表达以及ULF中凯莫瑞浓度在发情周期的不同阶段和怀孕早期受到激素环境影响的研究。本结果还表明凯莫瑞与猪生殖功能的调节有关。在周期的黄体期和孕产妇识别期间，在ULF中观察到最高的分析型脂肪因子浓度。这是第一项证明凯莫瑞系统在猪子宫，概念性子宫和滋养细胞中的表达以及ULF中凯莫瑞浓度在发情周期的不同阶段和怀孕早期受到激素环境影响的研究。本结果还表明凯莫瑞与猪生殖功能的调节有关。在周期的黄体期和孕产妇识别期间，在ULF中观察到最高的分析型脂肪因子浓度。这是第一项证明凯莫瑞系统在猪子宫，概念性子宫和滋养细胞中的表达以及ULF中凯莫瑞浓度在发情周期的不同阶段和怀孕早期受到激素环境影响的研究。本结果还表明凯莫瑞与猪生殖功能的调节有关。ULF中的chemerin和chemerin浓度在发情周期的不同阶段和怀孕初期受到激素环境的影响。本结果还表明凯莫瑞与猪生殖功能的调节有关。ULF中的chemerin和chemerin浓度在发情周期的不同阶段和怀孕初期受到激素环境的影响。目前的结果还表明，凯莫瑞与猪生殖功能的调节有关。