h-FIBER: Microfluidic Topographical Hollow Fiber for Studies of Glomerular Filtration Barrier.,ACS Central Science

当前位置： X-MOL 学术 › ACS Cent. Sci. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

h-FIBER: Microfluidic Topographical Hollow Fiber for Studies of Glomerular Filtration Barrier.
ACS Central Science ( IF 12.7 ) Pub Date : 2020-05-13 , DOI: 10.1021/acscentsci.9b01097
Ruoxiao Xie _{1,

2} , Anastasia Korolj _{2,

3} , Chuan Liu ₂ , Xin Song ₃ , Rick Xing Ze Lu ₂ , Boyang Zhang ₂ , Arun Ramachandran ₃ , Qionglin Liang ₁ , Milica Radisic _{2,

3}

Affiliation

Kidney-on-a-chip devices may revolutionize the discovery of new therapies. However, fabricating a 3D glomerulus remains a challenge, due to a requirement for a microscale soft material with complex topography to support cell culture in a native configuration. Here, we describe the use of microfluidic spinning to recapitulate complex concave and convex topographies over multiple length scales, required for biofabrication of a biomimetic 3D glomerulus. We produced a microfluidic extruded topographic hollow fiber (h-FIBER), consisting of a vessel-like perfusable tubular channel for endothelial cell cultivation, and a glomerulus-like knot with microconvex topography on its surface for podocyte cultivation. Meter long h-FIBERs were produced in microfluidics within minutes, followed by chemically induced inflation for generation of topographical cues on the 3D scaffold surface. The h-FIBERs were assembled into a hot-embossed plastic 96-well plate. Long-term perfusion, podocyte barrier formation, endothelialization, and permeability tests were easily performed by a standard pipetting technique on the platform. Following long-term culture (1 month), a functional filtration barrier, measured by the transfer of albumin from the blood vessel side to the ultrafiltrate side, suggested the establishment of an engineered glomerulus.

中文翻译：

h-FIBER：用于肾小球滤过屏障研究的微流体拓扑中空纤维。

肾脏芯片设备可能会彻底改变新疗法的发现。然而，制造 3D 肾小球仍然是一个挑战，因为需要具有复杂形貌的微型软材料来支持天然配置的细胞培养。在这里，我们描述了使用微流体旋转来重现多个长度尺度上的复杂凹凸形貌，这是仿生 3D 肾小球生物制造所需的。我们生产了一种微流体挤压形貌中空纤维（h-FIBER），由用于内皮细胞培养的血管状可灌注管状通道和用于足细胞培养的表面具有微凸形貌的肾小球样结组成。数米长的 h-FIBER 在微流体中在几分钟内生成，然后通过化学诱导膨胀在 3D 支架表面生成地形线索。 h-FIBER 被组装到热压花塑料 96 孔板中。通过平台上的标准移液技术可以轻松进行长期灌注、足细胞屏障形成、内皮化和渗透性测试。经过长期培养（1个月）后，通过白蛋白从血管侧到超滤液侧的转移来测量功能性过滤屏障，表明工程化肾小球的建立。

更新日期：2020-06-24

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11