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Pathogenicity and phylogenetic analysis of Fusarium oxysporum f. sp. capsici isolates from pepper in Turkey
Canadian Journal of Plant Pathology ( IF 1.6 ) Pub Date : 2019-08-16 , DOI: 10.1080/07060661.2019.1641749
Hacer Handan Altinok 1 , Gamze Yüksel 1 , Mahmut Alper Altinok 1
Affiliation  

Abstract The pathogenic Fusarium oxysporum species complex (FOSC) causes significant yield losses in the family Solanaceae including on tomato, eggplant and pepper in the Aegean and Mediterranean regions of Turkey. Fusarium wilt of pepper, caused by Fusarium oxysporum f. sp. capsici (Foc), has emerged recently in Turkey, where its significance has not been investigated thoroughly. In the present study, disease prevalence was determined with surveys conducted in 67 pepper-growing greenhouses and 56 pepper fields in the Aegean and Mediterranean regions in 2015 and 2016. In both years, about 32% of the greenhouses and 55% of the fields were found to be infested with the wilting disease. It was observed in a pathogenicity experiment that 64 isolates were pathogenic to pepper. In cross-inoculation experiments, these isolates did not cause wilting symptoms on tomato and eggplant and therefore were designated as Foc. Phylogenetic analyses were carried out with TEF-1α and actA DNA regions of a selected subset of 40 Foc isolates, along with non-host formae of F. oxysporum and outgroup Fusarium species. Sequence data for the TEF-1α and actA DNA regions displayed limited variation among Foc isolates and failed to distinguish reliably several formae speciales of F. oxysporum. The phylogenetic tree successfully separated the outgroup isolates. All Foc isolates were placed in the same branch, suggesting a single monophyletic group in the dendrogram. This is the first comprehensive study for pathogenic and phylogenetic analysis of Foc isolates from peppers in Turkey.

中文翻译:

尖孢镰刀菌的致病性和系统发育分析。sp. 从土耳其辣椒中分离出的辣椒

摘要 致病性尖孢镰刀菌 (Fusarium oxysporum species complex, FOSC) 导致茄科植物的显着减产,包括土耳其爱琴海和地中海地区的番茄、茄子和辣椒。辣椒枯萎病,由尖孢镰刀菌引起。sp. capsici (Foc),最近在土耳其出现,其重要性尚未得到彻底调查。在本研究中,2015 年和 2016 年在爱琴海和地中海地区的 67 个辣椒种植温室和 56 个辣椒田中进行了调查,确定了疾病流行率。在这两年中,大约 32% 的温室和 55% 的田地是发现感染了枯萎病。在致病性实验中观察到64株对辣椒具有致病性。在交叉接种实验中,这些分离株不会在番茄和茄子上引起萎蔫症状,因此被指定为 Foc。对选定的 40 个 Foc 分离株子集的 TEF-1α 和 actA DNA 区域以及尖孢镰刀菌和镰刀菌属物种的非寄主形式进行了系统发育分析。TEF-1α 和 actA DNA 区域的序列数据显示 Foc 分离株之间的变异有限,并且无法可靠地区分尖孢镰刀菌的几种特殊形式。系统发育树成功地分离了外群分离株。所有 Foc 分离株都放在同一分支中,表明树状图中有一个单系群。这是对土耳其辣椒 Foc 分离株的病原学和系统发育分析的首次综合研究。对选定的 40 个 Foc 分离株子集的 TEF-1α 和 actA DNA 区域以及尖孢镰刀菌和镰刀菌属物种的非寄主形式进行了系统发育分析。TEF-1α 和 actA DNA 区域的序列数据显示 Foc 分离株之间的变异有限,并且无法可靠地区分尖孢镰刀菌的几种特殊形式。系统发育树成功地分离了外群分离株。所有 Foc 分离株都放在同一分支中,表明树状图中有一个单系群。这是对土耳其辣椒 Foc 分离株的病原学和系统发育分析的首次综合研究。对选定的 40 个 Foc 分离株子集的 TEF-1α 和 actA DNA 区域以及尖孢镰刀菌和镰刀菌属物种的非寄主形式进行了系统发育分析。TEF-1α 和 actA DNA 区域的序列数据显示 Foc 分离株之间的变异有限,并且无法可靠地区分尖孢镰刀菌的几种特殊形式。系统发育树成功地分离了外群分离株。所有 Foc 分离株都放在同一分支中,表明树状图中有一个单系群。这是对土耳其辣椒 Foc 分离株的病原学和系统发育分析的首次综合研究。TEF-1α 和 actA DNA 区域的序列数据显示 Foc 分离株之间的变异有限,并且无法可靠地区分尖孢镰刀菌的几种特殊形式。系统发育树成功地分离了外群分离株。所有 Foc 分离株都放在同一分支中,表明树状图中有一个单系群。这是对土耳其辣椒 Foc 分离株的病原学和系统发育分析的首次综合研究。TEF-1α 和 actA DNA 区域的序列数据显示 Foc 分离株之间的变异有限,并且无法可靠地区分尖孢镰刀菌的几种特殊形式。系统发育树成功地分离了外群分离株。所有 Foc 分离株都放在同一分支中,表明树状图中有一个单系群。这是对土耳其辣椒 Foc 分离株的病原学和系统发育分析的首次综合研究。
更新日期:2019-08-16
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