ABSTRACT

We measured the induction and recovery time of anaesthetised olive flounder Paralichthys olivaceus with two concentrations of clove oil (25 and 50 μL/L), under the fluorescent lamp or green-wavelength light. In addition, we investigated their effects on the circadian rhythm after recovery from anaesthesia. We found that with the increase in anaesthesia concentration, induction time decreases and recovery time increases, in both light conditions. However, when anaesthetised with the same concentration of clove oil, induction time remained the same, but the recovery time was significantly decreased under green-wavelength light. The circadian rhythm was evaluated by quantifying the transcript levels of clock genes [period 2 (Per2) and cryptochrome1 (Cry1)] and activities of melatonin for 42 h. In the anaesthesia experimental group, the expression of the clock genes and activities of melatonin were significantly lower than that in the control group, and it was further lowered as the concentration of clove oil was increased. However, the anaesthetised group under green wavelength did not show a significant difference in circadian parameters compared to the control group, over time. This study provides the critical information on the use of clove oil anaesthesia in flounders and shows the effective recovery after anaesthesia upon exposure to green-wavelength light.

摘要

我们测量了麻醉橄榄比目鱼Paralichthys olivaceus的诱导和恢复时间在荧光灯或绿色波长光下，加入两种浓度的丁香油（25 和 50 μL/L）。此外，我们研究了它们对麻醉恢复后昼夜节律的影响。我们发现，在两种光照条件下，随着麻醉浓度的增加，诱导时间减少，恢复时间增加。然而，当用相同浓度的丁香油麻醉时，诱导时间保持不变，但在绿波长光下恢复时间明显缩短。通过量化时钟基因 [周期 2 (Per2) 和隐花色素 1 (Cry1)] 的转录水平和褪黑激素的活性 42 小时来评估昼夜节律。在麻醉实验组，生物钟基因的表达和褪黑激素的活性显着低于对照组，随着丁香油浓度的增加进一步降低。然而，随着时间的推移，与对照组相比，绿色波长下的麻醉组在昼夜节律参数方面没有显着差异。这项研究提供了有关在比目鱼中使用丁香油麻醉的关键信息，并显示了在暴露于绿色波长光后麻醉后的有效恢复。