ABSTRACT

In this study, effect high-energy diet with supplemented with copper and zinc on oxidative stress, leukocyte function and reproductive performance during the transition period in the crossbred cow under tropical settings. Advanced pregnant crossbred cows of two to four parity and lactation yield of >10 L/day were divided into three equal groups (n = 10 cow/group). Group I cows were fed basal diet to serve as control. Group II cows were supplemented with 20% additional concentrate, copper (15.7/kg DM) and zinc (22 mg/kg DM). Group III cows were provided with only energy in the form of 20% additional concentrate. Copper and zinc were supplemented from −4 before to +8 week of calving, whereas concentrate was given from +2 to +8 week after calving. Blood samples were collected at weekly interval from −4 to +8 week of calving. Group II had a significantly lower MDA, higher TAC and decreased activity of SOD and CAT than that of Group I (P < 0.05). Phagocytic activity and lymphocytic activity increased from 3 to 8 weeks postpartum (P < 0.05) in Group II and III indicating early restoration of leukocyte function. Resumption of postpartum oestrus was shorter in group II (P < 0.05).

摘要

在本研究中，在热带环境下，高能日粮补充铜和锌对杂交奶牛过渡期氧化应激、白细胞功能和繁殖性能的影响。将 2 至 4 胎和泌乳量 > 10 L/天的高级怀孕杂交奶牛分成 3 个相等的组（n= 10 头奶牛/组）。I组奶牛饲喂基础日粮作为对照。第 II 组奶牛补充了 20% 的额外浓缩物、铜（15.7/kg DM）和锌（22 mg/kg DM）。III 组奶牛仅以 20% 的额外浓缩物的形式提供能量。铜和锌从产犊前 -4 周到 +8 周补充，而精矿在产犊后 +2 到 +8 周给予。从产犊的-4 到+8 周，每周收集一次血样。Ⅱ组MDA显着低于Ⅰ组，TAC升高，SOD和CAT活性降低（P <0.05）。吞噬活性和淋巴细胞活性在产后 3 至 8 周增加（P< 0.05) 在 II 组和 III 组中表明白细胞功能的早期恢复。II组产后恢复发情时间较短（P < 0.05）。