ABSTRACT

Biological experiment was carried out with 240 chicks to study the effect of polyphenols on heat stress (HS) alleviation under different cereal sources in 2 × 3 factorial study. Six experimental groups consisted of two cereal groups (conventional and alternate cereals). Conventional has corn as sole cereal source, whereas in alternate group broken rice and sorghum were used. Each category was supplemented with 0, 50 and 100 ppm of polyphenols from pomegranate peel extract (PPE). Birds fed rice-sorghum diet with or without PPE had numerically higher values than corn counterpart. Cereal sources had a significant effect on core and comb temperature, as well as the respiration rate. HL ratio of birds exposed to HSwas altered significantly by dietary changes as well as the PPE supplementation. Birds fed with PPE had lower HL ratio than control groups. The mRNA expression level of HSP70 in the jejunum was significantly up-regulated in groups supplemented with PPP at 50 and 100 ppm in both types of cereal sources. There was higher serum HSP70 protein 1A in supplemented groups than their respective unsupplemented cereal groups. Supplementation of polyphenols as pomegranate peel extract at 50 ppm improved HSindicators, performance and antioxidant status with major activity in alternate cereal source (broken-rice-sorghum).

摘要

对240只雏鸡进行生物试验，以2×3因子研究不同谷类来源下多酚类物质对缓解热应激（HS）的影响。六个实验组由两个谷物组（常规谷物和替代谷物）组成。常规以玉米作为唯一的谷物来源，而在交替组中使用碎米和高粱。每个类别都添加了来自石榴皮提取物 (PPE) 的 0、50 和 100 ppm 多酚。喂食含有或不含 PPE 的水稻高粱饲料的鸟类在数值上高于玉米对应物。谷物来源对核心和梳状温度以及呼吸速率有显着影响。暴露于 HS 的家禽的 HL 比率因饮食变化和 PPE 补充剂而显着改变。饲喂 PPE 的鸡的 HL 比率低于对照组。在两种谷物来源中，在添加 50 和 100 ppm PPP 的组中，HSP70 的 mRNA 表达水平显着上调。补充组的血清 HSP70 蛋白 1A 高于各自未补充的谷物组。作为石榴皮提取物补充 50 ppm 的多酚可改善 HS 指标、性能和抗氧化状态，并在替代谷物来源（碎米高粱）中具有主要活性。