Abstract

Specificity Protein 3 (SP3) is a newly identified regulator of tumor growth and invasiveness in humans. In this study, we identified and characterized the function of duck SP3 (duSP3). The full-length cDNA sequence of the duSP3 gene was cloned via rapid amplification of cDNA ends. It contained 2468 nucleotides, including a 111 base pair (bp) 5′-untranslated region (UTR), 215 bp 3′-UTR, and 2142 bp open reading frame (ORF), which encoded a 713 amino acid (AA) strongly conserved with Avian SP3. Tissue specificity analysis demonstrated that duSP3 was constitutively expressed in the eight tissues tested: liver, spleen, lung, heart, kidney, thymus, breast, and leg; and low expression levels were observed in all tissues, except the spleen and thymus. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis revealed that duSP3 expression rapidly increased in vitro after stimulation with both the hepatitis virus (DHV-1) and polyriboinosinic polyribocytidylic acid (poly(I:C)). However, the expression under these treatments varied in kidney and liver tissues; in the liver, duSP3 increased significantly at 36 h after the DHV-1 treatment and peaked at 72 h after poly(I:C) stimulation. These results suggested that SP3 may play a positive role in immune responses against viral infections in ducks.

摘要

特异性蛋白 3 (SP3) 是一种新发现的人类肿瘤生长和侵袭性调节剂。在这项研究中，我们鉴定并表征了鸭SP3 ( duSP3 ) 的功能。duSP3基因的全长 cDNA 序列通过 cDNA 末端的快速扩增进行克隆。它包含 2468 个核苷酸，包括 111 个碱基对 (bp) 5'-非翻译区 (UTR)、215 bp 3'-UTR 和 2142 bp 开放阅读框 (ORF)，其编码了 713 个高度保守的氨基酸 (AA)与禽SP3。组织特异性分析表明duSP3在所测试的八种组织中组成型表达：肝、脾、肺、心脏、肾、胸腺、乳房和腿；在除脾脏和胸腺外的所有组织中均观察到低表达水平。定量实时聚合酶链反应 (qRT-PCR) 分析显示，在用肝炎病毒 (DHV-1) 和聚核糖肌苷酸 (poly(I:C)) 刺激后，duSP3在体外的表达迅速增加。然而，这些处理下的表达在肾脏和肝脏组织中有所不同。在肝脏中，duSP3在 DHV-1 治疗后 36 小时显着增加，并在聚（I:C）刺激后 72 小时达到峰值。这些结果表明SP3 可能在鸭对病毒感染的免疫反应中发挥积极作用。