In angiosperms, the usual storage tissue found in seeds is the endosperm, which originates at the characteristic event of the double fertilisation. The mature seeds of Gomortega keule (Molina) Baillon, in the monotypic family Gomortegaceae, contain a storage tissue composed of two distinct parts. This uncommon form suggests one of the parts may actually correspond to perisperm. In this work we aimed to combine genetic and histological observations to determine the endospermic origins of the two tissue parts. Seven microsatellite DNA loci were amplified and genotyped. Genetic profiles of tissue parts were grouped in expected classes following the model of the double fertilisation of angiosperms. The majority of observations matched those expected classes. Genotyping inconsistencies could be attributed to fruits that were mistakenly assigned to a maternal tree, or due to null alleles or mutations. In observations made from histological preparations, the outer part of the storage tissue represents a larger proportion in mature seeds than is found in young seeds, suggesting that the outer part of the storage tissue develops later than the inner part. The observations of the endosperm, a single cell layer of perisperm, and details of the seed coat, show that there was no evidence that the perisperm has any continuity with the storage tissue. This finding suggested a common endospermic origin for the two parts of the storage tissue. There was a cell layer in between the inner and outer part of the endosperm, which has not been previously reported, and it supports the finding of differentiation of the two endosperm parts. The pattern of two tissue parts in the endosperm of G. keule is a unique feature among angiosperm plants. The observations suggest early stages of evolution towards a greater histological differentiation of the endosperm parts in this species.

在被子植物中，种子中常见的贮藏组织是胚乳，胚乳起源于双重受精的特征事件。Gomortega keule的成熟种子（Molina）单性家族禾本科（Gomortegaceae）的Baillon包含由两个不同部分组成的储藏组织。这种罕见的形式表明其中一部分可能实际上对应于精子。在这项工作中，我们旨在结合遗传学和组织学观察来确定两个组织部位的胚乳起源。扩增了七个微卫星DNA基因座并进行了基因分型。按照被子植物双重受精的模型，将组织部位的遗传概况按预期类别分组。大多数观察结果与那些预期类别相符。基因分型不一致可能归因于错误分配给母树的果实，或者归因于等位基因无效或突变。从组织学准备的观察中，存储组织的外部部分在成熟种子中所占的比例比年轻种子中所占的比例大，这表明存储组织的外部部分要比内部部分晚。对胚乳，胚乳的单个细胞层以及种皮细节的观察表明，没有证据表明胚乳与贮藏组织具有任何连续性。这一发现提示了储存组织的两个部分的共同的胚乳起源。在胚乳的内部和外部之间有一个细胞层，这以前没有报道过，它支持发现两个胚乳部分的分化。胚乳中两个组织部位的模式。对胚乳，胚乳的单个细胞层以及种皮细节的观察表明，没有证据表明胚乳与贮藏组织具有任何连续性。这一发现提示了储存组织的两个部分的共同的胚乳起源。在胚乳的内部和外部之间有一个细胞层，以前没有报道过，它支持发现两个胚乳部分的分化。胚乳中两个组织部位的模式。对胚乳，胚乳的单个细胞层以及种皮细节的观察表明，没有证据表明胚乳与贮藏组织具有任何连续性。这一发现提示了储存组织的两个部分的共同的胚乳起源。在胚乳的内部和外部之间有一个细胞层，以前没有报道过，它支持发现两个胚乳部分的分化。胚乳中两个组织部位的模式。在胚乳的内部和外部之间有一个细胞层，以前没有报道过，它支持发现两个胚乳部分的分化。胚乳中两个组织部位的模式。在胚乳的内部和外部之间有一个细胞层，以前没有报道过，它支持发现两个胚乳部分的分化。胚乳中两个组织部位的模式。G. keule是被子植物中的独特功能。观察结果表明，该物种的胚乳部分向更大的组织学分化方向发展的早期阶段。