ABSTRACT Hexaploid Caucasian clover was grown in soil sampled at three New Zealand South Island high country sites to which specific rhizobium inoculum had been added with sowing of the legume in 1975, 1992 and 1997; two sites on the Lincoln University farm sown with inoculated Caucasian clover in 2012 and 2013; and six sites not sown with the crop. Caucasian clover nodulated in soil from all sites sown with inoculated Caucasian clover but did not nodulate in soils from the other sites. Rhizobial isolates from plants in each soil where nodulation occurred showed the same genetic profile, and gave a similar increase in growth of Caucasian clover in low nitrogen soil, as the recommended inoculum for hexaploid Caucasian clover. Caucasian clover specific rhizobia can persist and retain their effectiveness for at least 42 years in New Zealand South Island low fertility, high country soils and 5 years in high fertility soils.

中文翻译：

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高加索三叶草（Trifolium ambiguum）特异性根瘤菌在新西兰南岛的低肥力和高肥力土壤中持续存在

摘要 六倍体高加索三叶草生长在新西兰南岛三个高原地区采样的土壤中，在 1975、1992 和 1997 年播种豆科植物时，在这些地点添加了特定的根瘤菌接种物；2012 年和 2013 年，林肯大学农场的两个地点播种了接种的高加索三叶草；和六个未播种作物的地点。高加索三叶草在所有接种了高加索三叶草的地点的土壤中都形成了根瘤，但在其他地点的土壤中没有形成根瘤。从发生根瘤的每个土壤中的植物中分离出的根瘤菌显示出相同的遗传特征，并且在低氮土壤中高加索三叶草的生长增加类似，作为六倍体高加索三叶草的推荐接种物。