ABSTRACT Globalization of seafood product marketing caused the increase of request of an effective fish traceability that enhances the consumer confidence in food safety. In this study, an integrated multi analytical approach based on two different and independent analytical techniques (carbon and nitrogen stable isotopes and fatty acids analysis) was applied in order to identify different fish species and trace their geographical provenience. The investigation was focused on four species (Thunnus thynnus, Thunnus alalunga, Auxis rochei and Scomber scombrus) belonging to the Scombridae family. The DNA barcoding method confirmed genus and species for S. scombrus and A. rochei, but only genus for T. alalunga and T. thynnus. Carbon and nitrogen stable isotopes results evidenced different fish diets and trophic positions, whereas fatty acids analysis displayed that the unsaturated prevailed (∼60 %) over the saturated compounds with a variation among the species and the geographical area in particular for docosahexaenoic and eicosapentaenoic acids percentage. The principal component analysis applied to stable isotopes and fatty acids evidenced a good discrimination among species and their geographical catching area. This multi-disciplinary analytical approach could represent a promising tool to identify the commercial fish and trace their origin in order to guarantee the health of consumers.

中文翻译：

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解决鲭科种内地理可追溯性的初步多分析方法

摘要 海鲜产品营销的全球化导致对有效鱼类可追溯性的要求增加，从而增强了消费者对食品安全的信心。在这项研究中，应用基于两种不同且独立的分析技术（碳和氮稳定同位素和脂肪酸分析）的综合多分析方法来识别不同的鱼类并追踪其地理来源。调查的重点是属于 Scombridae 科的四个物种（Thunnus thynnus、Thunnus aalunga、Auxis rochei 和 Scomber scombrus）。DNA条形码方法确认了S. scombrus和A. rochei的属和种，但仅确认了T. alalunga和T. thynnus的属。碳和氮稳定同位素结果证明了不同的鱼类饮食和营养位置，而脂肪酸分析显示不饱和化合物占优势（~60%），在物种和地理区域之间存在差异，特别是二十二碳六烯酸和二十碳五烯酸百分比。应用于稳定同位素和脂肪酸的主成分分析证明了物种之间的良好区分及其地理捕获区域。这种多学科分析方法可能是一种很有前途的工具，可以识别商业鱼类并追踪其来源，以保证消费者的健康。适用于稳定同位素和脂肪酸的主成分分析证明了物种之间的良好区分及其地理捕获区域。这种多学科分析方法可能是一种很有前途的工具，可以识别商业鱼类并追踪其来源，以保证消费者的健康。应用于稳定同位素和脂肪酸的主成分分析证明了物种之间的良好区分及其地理捕获区域。这种多学科分析方法可能是一种很有前途的工具，可以识别商业鱼类并追踪其来源，以保证消费者的健康。