ABSTRACT Plantacyanin (PLC) plays an important role in plant growth, development, and responses to metal cation stress. However, the function of PLC in tea remains unclear. In this study, three PLC genes were identified from the tea plant. Protein sequences and structure analyses indicated that the CsPLCs contained four conserved sites, and furthermore, promoter analysis showed that the genes were involved in growth and development, signal transduction, and stress responses of the tea plant. Quantitative real-time PCR analyses revealed that the CsPLCs exhibited organ-specific expression. CsPLC-1 was highly expressed in roots and stems, CsPLC-2 was highest expressed in stems, and CsPLC-3 in leaves. Additionally, Cu2+, Al3+, and NaCl stress strongly influence the expression of CsPLC-3. Subcellular localisation assays revealed that CsPLC-3 was the plasma membrane and nuclear localised protein. Moreover, the CsPLC-3 gene enhanced Cu2+, Al3+, and NaCl tolerance in Saccharomyces cerevisiae and was significantly affected by pH.

中文翻译：

---

油茶植物花青素（PLC）基因的鉴定分析及酵母中CsPLC-3的功能鉴定

摘要 植物花青素 (PLC) 在植物生长、发育和对金属阳离子胁迫的响应中起着重要作用。然而，PLC在茶叶中的作用尚不清楚。在这项研究中，从茶树中鉴定了三个 PLC 基因。蛋白质序列和结构分析表明，CsPLCs 含有四个保守位点，此外，启动子分析表明这些基因参与了茶树的生长发育、信号转导和逆境响应。定量实时 PCR 分析显示 CsPLCs 表现出器官特异性表达。CsPLC-1在根和茎中高表达，CsPLC-2在茎中表达最高，CsPLC-3在叶中表达最高。此外，Cu2+、Al3+ 和NaCl 胁迫强烈影响CsPLC-3 的表达。亚细胞定位分析表明，CsPLC-3 是质膜和核定位蛋白。此外，CsPLC-3 基因增强了酿酒酵母对 Cu2+、Al3+ 和 NaCl 的耐受性，并受 pH 显着影响。