ABSTRACT Oil bodies (OBs) are organelles of lipid storage in plants. In order to clarify the properties of OBs development in herbaceous peony (Paeonia lactiflora Pall.) seeds, this research had studied anatomical structures, particle size, zeta potential, the stability of OBs and characteristics of oleosin gene (OLE). It showed that OB particle size increased gradually during seed development, OBs accumulated in the endosperm cells, and their zeta potential firstly decreased and then increased. Temperature had not affected the stability of OBs, they could be maintained stably under the conditions of neutral and alkaline pH and less than 50 mM NaCl. Subsequently, P. lactiflora OLE was isolated, it had 824 bp in full-length and a 435-bp open reading frame encoding 144 amino acids with isoelectric point 9.40, which had three transmembrane domain and did not has a signal peptide, and belonged to the stable protein. Alpha helix was the main part of the secondary structure, no glycosylation site and 14 potential phosphorylation sites were found. Additionally, the express level of OLE initially increased and then decreased, and reached the highest on 60 days after flowering. These results could contribute to understand the properties of OBs development and characteristics of OLE in P. lactiflora seeds.

中文翻译：

---

芍药(Paeoinia lactiflora Pall.)种子油体发育特性及OLE基因特征

摘要油体（OBs）是植物脂质储存的细胞器。为阐明牡丹(Paeonia lactiflora Pall.)种子中OBs发育的特性，本研究对OBs的解剖结构、粒径、zeta电位、OBs的稳定性和油质蛋白基因(OLE)的特性进行了研究。结果表明，在种子发育过程中OB粒径逐渐增大，OBs在胚乳细胞中积累，其zeta电位先降低后升高。温度对OBs的稳定性没有影响，在中性和碱性pH和低于50 mM NaCl的条件下，它们都可以稳定保持。随后分离到P.lactiflora OLE，全长824 bp，开放阅读框435 bp，编码144个氨基酸，等电点为9.40，具有三个跨膜结构域，不含信号肽，属于稳定蛋白。α螺旋是二级结构的主要部分，没有发现糖基化位点和14个潜在的磷酸化位点。此外，OLE的表达水平先升高后降低，在开花后60天达到最高。这些结果有助于了解 OB 发育的特性和 P.lactiflora 种子中 OLE 的特性。