ABSTRACT Vascular streak dieback is a serious disease of cocoa throughout the world and breeding of resistant varieties is the most reliable management strategy. Molecular markers linked with resistance enable marker-assisted breeding by tracking the resistance genes in each generation. To identify the markers linked with resistance, four accessions each of highly resistant, highly susceptible and partially resistant categories were screened with ISSR and SSR primers. Of the 71 ISSR and 46 SSR primers used, five ISSR primers UBC811, UBC815, UBC826, UBC857 and UBC866 have yielded reproducible markers for resistance. Cloning and sequencing of UBC857 marker showed 94% identity with SSR clone mTcCIR42. Screening of genotypes with mTcCIR42 primers generated distinct markers for resistance. Identified ISSR markers represent at least three different genes. Results were further validated using the resistant and susceptible progenies and are being used in marker-assisted selection for VSD resistance in cocoa.

中文翻译：

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微卫星和微卫星间标记与可可（Theobroma cacao L.）对血管条纹枯死的抗性有关

摘要 脉管条纹枯死是世界范围内可可的严重病害，培育抗病品种是最可靠的管理策略。与抗性相关的分子标记通过跟踪每一代的抗性基因来实现标记辅助育种。为了鉴定与抗性相关的标记，用ISSR和SSR引物筛选了高抗性、高度敏感和部分抗性类别的四个种质。在使用的 71 个 ISSR 和 46 个 SSR 引物中，五个 ISSR 引物 UBC811、UBC815、UBC826、UBC857 和 UBC866 产生了可重复的抗性标记。UBC857 标记的克隆和测序显示与 SSR 克隆 mTcCIR42 具有 94% 的同一性。用 mTcCIR42 引物筛选基因型产生了不同的抗性标记。已鉴定的 ISSR 标记代表至少三个不同的基因。使用抗性和易感后代进一步验证了结果，并用于可可中 VSD 抗性的标记辅助选择。