ABSTRACT Heat shock Protein (HSP) expression is the plants’ responses to high temperature. We analysed the HSP70 gene family in Agave sisalana transcriptome to understand its expression under heat stress. A total of 167 genes were identified as heat shock protein genes. Among them; 74, 39 and 25 belong to the HSP70, HSP20 HSP90 respectively and multiple sequence alignment of conserved domain proteins of AsHSP70 showed that they are highly conserved. Fifty-two AsHSPs were classified into three major classes according to their structural characteristics. Phylogenetic analysis indicated that orthologs from Agave deserti and Agave tequilana species were distributed among the three classes; which revealed the diverse expression of HSP70 gene family. In silico subcellular localisation of AsHSP, suggested that its interaction is quite diverse in target cells and active signalling pathways. Transcripts identified and named as B1, B3, B5, B7, B9, B11, B13, B15, B17 and B19 were validated through Nested PCR. Significant difference in the expression pattern of target gene was revealed after normalisation with GAPDH. Gene expression was up-regulated with some exceptions as down-regulated. In silico analysis of HSP70 gene family identified from A. sisalana suggests that genetic transformation of this gene will induce heat tolerance in transgenic crops.

中文翻译：

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热应激条件下热休克蛋白基因（HSP70）家族及其在龙舌兰中表达的鉴定与表征

摘要 热休克蛋白（HSP）的表达是植物对高温的反应。我们分析了龙舌兰转录组中的 HSP70 基因家族，以了解其在热应激下的表达。共有167个基因被鉴定为热休克蛋白基因。他们之中; 74、39和25分别属于HSP70、HSP20和HSP90，AsHSP70保守结构域蛋白的多序列比对表明它们是高度保守的。52 种 AsHSP 根据其结构特征分为三大类。系统发育分析表明，龙舌兰和龙舌兰龙舌兰属的直系同源物分布在三个纲中；这揭示了 HSP70 基因家族的多样化表达。在 AsHSP 的计算机亚细胞定位中，表明它的相互作用在靶细胞和活跃的信号通路中非常多样化。鉴定并命名为 B1、B3、B5、B7、B9、B11、B13、B15、B17 和 B19 的转录本通过嵌套 PCR 进行验证。用 GAPDH 归一化后，发现靶基因表达模式存在显着差异。基因表达被上调，但有一些例外被下调。从 A. sisalana 鉴定的 HSP70 基因家族的计算机分析表明，该基因的遗传转化将诱导转基因作物的耐热性。基因表达被上调，但有一些例外被下调。从 A. sisalana 鉴定的 HSP70 基因家族的计算机分析表明，该基因的遗传转化将诱导转基因作物的耐热性。基因表达被上调，但有一些例外被下调。从 A. sisalana 鉴定的 HSP70 基因家族的计算机分析表明，该基因的遗传转化将诱导转基因作物的耐热性。