ABSTRACT A wide range of factors including biotic and abiotic stresses influence anthocyanin accumulation in plants. In this study, experiments were designed to measure the effects of nutrient depletion on anthocyanin accumulation in 3-week old juvenile Brassica oleracea seedlings of the red cabbage cultivar ‘Futurima’ and the red kale cultivar ‘Redbor’ that were grown in a hydroponic system. The effects of the depletion of single nutrients were compared to standard fertigation conditions. Plants were grown for 2 weeks in the absence of nitrogen accumulated higher anthocyanin levels than in all other treatments in both ‘Futurima’ and ‘Redbor’. Nutrient depletion treatments significantly influenced the percentage of anthocyanin diacylation in ‘Futurima’, but not in ‘Redbor’. A time-course experiment revealed that juvenile cabbage and kale seedlings accumulated maximal anthocyanin concentrations after 18 days without a supply of nitrogen. ’Redbor’ chromatograms were influenced by the localisation of anthocyanins in plant tissues, while nitrogen depletion treatments significantly increased anthocyanin concentrations in stems and leaves. In both greenhouse and growth chamber conditions, nitrogen depletion treatments in ‘Redbor’ significantly increased the percentage of diacylated anthocyanin compounds compared to control treatments in stems but not in leaves. These findings will help to optimise anthocyanin production in juvenile Brassica oleracea plants.

中文翻译：

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施肥对甘蓝花色苷积累和酰化的影响

摘要 包括生物和非生物胁迫在内的多种因素会影响植物中花青素的积累。在这项研究中，实验旨在测量营养消耗对在水培系统中生长的红甘蓝品种 'Futurima' 和红色羽衣甘蓝品种 'Redbor' 的 3 周龄芸苔幼苗中花青素积累的影响。将单一养分消耗的影响与标准施肥条件进行比较。植物在没有氮的情况下生长 2 周，在 'Futurima' 和 'Redbor' 中积累的花青素水平高于所有其他处理。营养消耗处理显着影响了 'Futurima' 中花青素二酰化的百分比，但不影响 'Redbor'。一个时间进程实验表明，幼白菜和羽衣甘蓝幼苗在没有氮供应的情况下 18 天后积累了最大的花青素浓度。'Redbor' 色谱图受植物组织中花青素定位的影响，而氮消耗处理显着增加了茎和叶中的花青素浓度。在温室和生长室条件下，与茎中的对照处理相比，'Redbor' 中的氮耗竭处理显着增加了二酰化花青素化合物的百分比，但在叶子中则没有。这些发现将有助于优化甘蓝幼苗中花青素的生产。'Redbor' 色谱图受植物组织中花青素定位的影响，而氮消耗处理显着增加了茎和叶中的花青素浓度。在温室和生长室条件下，与茎中的对照处理相比，'Redbor' 中的氮耗竭处理显着增加了二酰化花青素化合物的百分比，但在叶子中则没有。这些发现将有助于优化甘蓝幼苗中花青素的生产。'Redbor' 色谱图受植物组织中花青素定位的影响，而氮消耗处理显着增加了茎和叶中的花青素浓度。在温室和生长室条件下，与茎中的对照处理相比，'Redbor' 中的氮耗竭处理显着增加了二酰化花青素化合物的百分比，但在叶子中则没有。这些发现将有助于优化甘蓝幼苗中花青素的生产。与茎中的对照处理相比，'Redbor' 中的氮耗竭处理显着增加了二酰化花青素化合物的百分比，但在叶子中则没有。这些发现将有助于优化甘蓝幼苗中花青素的生产。与茎中的对照处理相比，'Redbor' 中的氮耗竭处理显着增加了二酰化花青素化合物的百分比，但在叶子中则没有。这些发现将有助于优化甘蓝幼苗中花青素的生产。