Abstract

The establishment of seed banks is essential for the conservation of cultivated and wild plant species. Cattleya species are economically important, but many of them are at risk of extinction. In this study, we aimed to evaluate the effects of storage temperature on the physiological quality of seeds of seven congeneric species of diverse biomes from the UNOESTE-OSSSU seed bank and the lipid composition. Seeds were split into sub samples, pre-conditioned to 0.03 g H₂O g DW⁻¹ for a week over a supersaturated solution of lithium chloride at 23 ± 2 °C and maintained in rubber-sealed vessels for nine months under four storage temperatures: room temperature (23 ± 2 °C), 5 °C, –18 °C and –196 °C. Germination, the germination speed index, and viability based on a tetrazolium test were evaluated at three-month intervals. Lipid profiles were determined by GC-MS in the original seed lots. Different lipid profiles were found among species. Orchid seeds with a low water content could be stored for a short period in all conditions studied and were considered orthodox short-lived seeds. Cattleya rupestris seeds were more tolerant than the congeners to deterioration under all the storage conditions.

摘要

建立种子库对于保护栽培和野生植物物种至关重要。卡特兰物种在经济上很重要，但其中许多有灭绝的危险。在这项研究中，我们旨在评估贮藏温度对UNOESTE-OSSSU种子库中七个不同生物群落的同系物种种子的生理品质和脂质组成的影响。将种子分成子样品，预先调节至0.03 g H ₂ O g DW ^-1在23±2°C的过饱和氯化锂溶液中放置一周，并在以下四个存储温度下在橡胶密封的容器中保持9个月：室温（23±2°C），5°C，–18°C和–196°C。在三个月的间隔内评估基于四唑鎓测试的发芽，发芽速度指数和生存力。通过GC-MS测定原始种子批次中的脂质谱。在物种之间发现了不同的脂质谱。含水量低的兰花种子可以在所有研究条件下短时间保存，被认为是正统的短寿命种子。在所有存储条件下，Cattleya rupestris种子比同类植物更耐腐。