Abstract

Saffron is one of the most expensive spices and is rich in pharmacological and biological active ingredients. We explored the community structure and diversity of the fungi growing on the surface of saffron corms having different growth characteristics. Thirty corm samples having different growth characteristics, i.e., infected, unable to germinate, able to germinate but not bloom, able to germinate and produce one flower per corm, and able to germinate and produce two flowers per corm, were collected from South Tai Lake Agricultural Park, Huzhou central hospital. The next-generation sequencing (NGS) method was employed to detect the internal transcribed spacer (ITS) ribosomal RNA in fungus. The community structure of fungi on the surface of corms having different growth characteristics was described using bioinformatics tools, and the specific fungus on the corm surface was identified. The fungi on the surface of corms exhibited different developmental features. The abundance of Trichocomaceae and Talaromyces was high in infected corms, whereas that of Aspergillaceae was high in corms that were unable to germinate. Penicillium and Dothideomycetes were observed in corms that could germinate but not bloom, and in corms that could germinate and produce two flowers per corm, respectively. Differences were observed in Cladosporium, Lambertella, Passalora, Penicillium, and Talaromyces among the five groups at the genus level. An understanding of the fungal diversity on the corm surface might provide useful information for improving germination and blooming of saffron corms.

摘要

藏红花是最昂贵的香料之一，富含药理和生物活性成分。我们探索了具有不同生长特性的藏红花球茎表面生长的真菌的群落结构和多样性。从南太湖收集了三十个具有不同生长特性的球茎样品，即被感染，不能发芽，能够发芽但不能开花，每个球茎能够发芽并产生一朵花，并且每个球茎能够发芽并产生两朵花。湖州中心医院农业园区。下一代测序（NGS）方法用于检测真菌中的内部转录间隔区（ITS）核糖体RNA。使用生物信息学工具描述了具有不同生长特性的球茎表面真菌的群落结构，并鉴定了球茎表面的特定真菌。球茎表面的真菌表现出不同的发育特征。丰富的滴虫科和伞形科在受感染的球茎中含量较高，而曲霉科在不能发芽的球茎中含量较高。在可能发芽但不能开花的球茎中以及在可能发芽并每个球茎产生两朵花的球茎中观察到青霉菌和十二碳菌。中观察到的差异枝孢，Lambertella，Passalora，青霉，和踝节菌属在属水平的五组之一。对球茎表面真菌多样性的了解可能为改善藏红花球茎的发芽和开花提供有用的信息。