Abstract

Melaleuca styphelioides is considered as medicinal plant. This study was carried out to evaluate for the first time the phytochemical composition and to compare the antifungal activities of essential oil (EOs), methanol and aqueous extracts of M. styphelioides Sm. leaves against three fungi (Aspergillus niger, Rhizopus nigricans and Penicillium digitatum). A total of 10 components of the EO were identified, with the principal compound being methyl eugenol (87.2%). Results of the phytochemical analysis of leaves extract exhibited the presence of different phytoconstituents (phenolic compounds, flavonoids, tannins and anthocyanins). Volatile and non-volatile extracts were found to express dose-dependent inhibition against all tested fungi. Indeed, the EO oil showed significant inhibition of fungal growth and the IC₅₀ was 2.08 µL/mL for A. niger indicating that M. styphelioides leaf EO was particularly effective against this pathogen. The most susceptible species for the aqueous extract was P. digitatum (IC₅₀= 9.54 mg/mL) whereas R. nigricans was found to be more susceptible to the methanolic extract (IC₅₀= 8.31 mg/mL). Thus, the EO and aqueous as well as methanol extracts of M. styphelioides leaves possess antifungal activity and hence, it can be suggested for use in the food or pharmaceutical industries as an alternative to chemical preservatives.

摘要

千层白千层被认为是药用植物。进行了这项研究，以第一次评估其化学组成，并比较香精支原体Sm的精油（EOs），甲醇和水提取物的抗真菌活性。叶对三种真菌（黑曲霉，黑根霉和指状青霉）。总共鉴定出EO的10个成分，主要化合物为甲基丁香酚（87.2％）。叶片提取物的植物化学分析结果显示存在不同的植物成分（酚类化合物，类黄酮，单宁和花色苷）。发现挥发性和非挥发性提取物对所有测试真菌均表现出剂量依赖性抑制作用。实际上，EO油显示出对真菌生长的显着抑制作用，而黑曲霉的IC ₅₀为2.08 µL / mL，表明鼠疫杆菌叶EO对这种病原体特别有效。水提取物最易感的物种是指状假单胞菌（IC ₅₀ = 9.54 mg / mL），而黑纹罗勒菌被发现对甲醇提取物更敏感（IC ₅₀ = 8.31 mg / mL）。因此，苯乙烯类固氮菌叶子的EO和水提取物以及甲醇提取物均具有抗真菌活性，因此，可以建议在食品或制药行业中用作化学防腐剂的替代品。