Abstract

Growth and production of carotenoid in three Dunaliella species (Dunaliella salina (Dunal) Teodoresco, Dunaliella bardawil Ben-Amotz & Avron and Dunaliella sp.) were investigated using flat-plate photobioreactors in outdoor conditions with two optical paths (3 cm and 5 cm). The experiment was conducted in duplicate and lasted four weeks during which light intensity, temperature, pH and optical density were checked daily. The pigment production (total carotenoid and chlorophyll a) was monitored every two days. To induce an additional stress besides temperature and light intensity, two different salt concentrations were used, i.e. 6% and 8% NaCl. The highest growth in all treatment groups was noticed for Dunaliella sp. followed by D. bardawil and D. salina. D. salina produced a higher content of carotenoid concentrations corresponding to 5 cm/8% and 5 cm/6% groups; 779.102 ± 0.434 μg.mL⁻¹ and 694.326 ± 0.098 μg.mL⁻¹ were registered at the end of the experiment. The same species had also greater content of β-carotene.

摘要

使用平板光生物反应器，在室外条件下，通过两条光路（3 cm和5 cm），研究了三种杜氏藻（杜氏杜氏藻，Duodorella Teodoresco，杜氏杜鹃Ben-Amotz＆Avron和杜氏藻）中类胡萝卜素的生长和产生。 。实验进行两次，并持续四个星期，在此期间每天检查光强度，温度，pH和光密度。每两天监测一次色素生成（总类胡萝卜素和叶绿素a）。为了引起除温度和光强度之外的额外应力，使用了两种不同的盐浓度，即6％和8％NaCl。在所有治疗组中，增长最快的是杜氏藻 其次是D. bardawil和D. salina。D. salina产生较高含量的类胡萝卜素，对应于5 cm / 8％和5 cm / 6％的组；779.102±0.434μg.mL ^-1和694.326±0.098μg.mL ^-1均在实验结束登记。同一物种的β-胡萝卜素含量也更高。