Abstract

The effects of drought stress on the morphological and physiological features of highbush blueberry Brigitta Blue plants were investigated. The plants were propagated conventionally by stem cutting (SC) and tissue culture (TC). Micropropagated plants originating from axillary (TC-Ax) and adventitious (TC-Ad) shoots were separated. An additional group consisted of plants propagated several times by stem cuttings, derived from TC mother plants (TC/SC). Different concentrations of polyethylene glycol (PEG) (3% and 7%) were applied to induce drought stress. Parameters such as dry and fresh weight, water content, shoot elongation as well as chlorophyll fluorescence and content were measured. The effect of PEG treatment varied in plants of different origin. Shoot elongation was mainly inhibited in SC plants, whereas TC/SC plants exhibited a stronger reduction of photosystem II efficiency. Fm and Fv differences between TC plants propagated by axillary or adventitious shoots were also observed under control conditions. These epigenotypes also differed in shoot elongation under 7% PEG treatment. The obtained results confirmed the influence of the propagation method of highbush blueberry plants not only on morphological traits, but also on the physiological status.

摘要

研究了干旱胁迫对高丛蓝莓Brigitta Blue植物形态和生理特性的影响。常规地通过茎切（SC）和组织培养（TC）繁殖植物。分离了源自腋生（TC-Ax）和不定形（TC-Ad）芽的微繁植物。另一类是由TC母本植物（TC / SC）衍生的通过插条繁殖了几次的植物。应用不同浓度的聚乙二醇（PEG）（3％和7％）诱导干旱胁迫。测量诸如干重和鲜重，水含量，枝条伸长率以及叶绿素荧光和含量等参数。PEG处理的效果在不同来源的植物中有所不同。茎伸长主要在SC植物中受到抑制，而TC / SC植物表现出的光系统II效率降低幅度更大。在对照条件下，还观察到通过腋生或不定芽繁殖的TC植物之间的Fm和Fv差异。这些表型在7％PEG处理下的茎伸长也不同。所得结果证实了高丛蓝莓植物的繁殖方法不仅对形态性状的影响，而且对生理状态的影响。