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Isolation and Molecular Identification of Fungi with Magnesite Enrichment Potential from KÜMAŞ Quarries in Turkey
Geomicrobiology Journal ( IF 2.3 ) Pub Date : 2020-04-15 , DOI: 10.1080/01490451.2020.1751749
Medine Gulluce 1 , Selin Dogan 2 , Mehmet Karadayi 1 , Burak Alaylar 3 , Taha Yasin Koc 2 , Selma Sezen 4
Affiliation  

Abstract Magnesite is an important raw material used in various industrial applications, especially the production of high-temperature resistant materials. Due to its high reactant nature, magnesite ore is not found in pure form and it contains a great variety of pollutants such as calcium compounds, which restrict its use when exceeding 1% of the ore. Thus, the development of efficient strategies for the removal of pollutants remains a crucial step for magnesite utilization. In this regard, our present work was conducted to isolate and identify active fungal strains that remove calcium pollutants without changing the main magnesium content of the ore. For this aim, magnesite ore samples were collected from two quarries (Turanocağı and Ortaocak) of KÜMAŞ Magnesite Inc. and fungal isolation studies were done by using the ore’s flora. Active isolates were chosen according to their CaCO3 and MgCO3 dissolving capabilities and identified by using conventional light microscopy and molecular characterization techniques. 71 fungal isolates were obtained from the isolation step and 14 of them were chosen as active isolates that solve calcium compounds while not affecting the magnesium component. The data of the microscopic examination and 18S rDNA gene sequence analysis showed that 14 active strains with magnesite enrichment potential grouped in Aspergillus alliaceus (3), Aspergillus flavus (2), Aspergillus leporis (1), Aspergillus nomius (1), Fusarium tricinctum (2), Penicillium chrysogenum (1) and Penicillium sp. (4).

中文翻译:

来自土耳其 KÜMAŞ 采石场的具有菱镁矿富集潜力的真菌的分离和分子鉴定

摘要 菱镁矿是一种重要的原料,用于各种工业应用,特别是耐高温材料的生产。由于其高反应物性质,菱镁矿石并非以纯形式存在,并且含有多种污染物,如钙化合物,当超过矿石的 1% 时,限制其使用。因此,开发有效的污染物去除策略仍然是菱镁矿利用的关键步骤。在这方面,我们目前的工作是分离和鉴定可去除钙污染物而不改变矿石的主要镁含量的活性真菌菌株。为此,从 KÜMAŞ Magnesite Inc. 的两个采石场(Turanocağı 和 Ortaocak)收集了菱镁矿样品,并使用矿石的菌群进行了真菌分离研究。根据其 CaCO3 和 MgCO3 溶解能力选择活性分离株,并使用常规光学显微镜和分子表征技术进行鉴定。从分离步骤中获得了 71 种真菌分离物,其中 14 种被选为在不影响镁成分的情况下溶解钙化合物的活性分离物。镜检和18S rDNA基因序列分析数据显示,14株具有菱镁矿富集潜力的活性菌株归于葱白曲霉(3)、黄曲霉(2)、麻风曲霉(1)、诺米斯曲霉(1)、镰刀菌( 2)、Penicillium chrysogenum (1) 和 Penicillium sp。(4). 从分离步骤中获得了 71 种真菌分离物,其中 14 种被选为在不影响镁成分的情况下溶解钙化合物的活性分离物。镜检和18S rDNA基因序列分析数据显示,14株具有菱镁矿富集潜力的活性菌株归于葱白曲霉(3)、黄曲霉(2)、麻风曲霉(1)、诺米斯曲霉(1)、镰刀菌( 2)、Penicillium chrysogenum (1) 和 Penicillium sp。(4). 从分离步骤中获得了 71 种真菌分离物,其中 14 种被选为在不影响镁成分的情况下溶解钙化合物的活性分离物。镜检和18S rDNA基因序列分析数据显示,14株具有菱镁矿富集潜力的活性菌株归于葱白曲霉(3)、黄曲霉(2)、麻风曲霉(1)、诺米斯曲霉(1)、镰刀菌( 2)、Penicillium chrysogenum (1) 和 Penicillium sp。(4). 镜检和18S rDNA基因序列分析数据显示,14株具有菱镁矿富集潜力的活性菌株归于葱白曲霉(3)、黄曲霉(2)、麻风曲霉(1)、诺米斯曲霉(1)、镰刀菌( 2)、Penicillium chrysogenum (1) 和 Penicillium sp。(4). 镜检和18S rDNA基因序列分析数据显示,14株具有菱镁矿富集潜力的活性菌株归于葱白曲霉(3)、黄曲霉(2)、麻风曲霉(1)、诺米斯曲霉(1)、镰刀菌( 2)、Penicillium chrysogenum (1) 和 Penicillium sp。(4).
更新日期:2020-04-15
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