Abstract

Aim of the study: Dopaminergic system in the amygdala and Prefrontal Cortex (PFC) influences fear and anxiety through Dopaminergic Receptors (DR), especially DR-D1 and DR-D2. Transcription Factor EB (TFEB) is an upstream protein of the autophagy-lysosome pathway.

Materials and methods

In this study we have evaluated the behavioral mood variations, and expression of DR-D2 and TFEB genes in the amygdala and PFC of aggressive male rats’ offspring.

Results

Anxiety and depression-like behaviors were observed, but intra-ventricle injection of DR-D2 antagonist (Sulpiride) has shown to be efficient in reducing negative behavioral changes in offspring. Furthermore, DR-D2 gene expression was increased in the amygdala and PFC of aggressive male rats’ offspring, which the injection of Sulpiride decreased it significantly. TFEB gene expression was also decreased in the amygdala and PFC of aggressive male rats’ offspring, but the blockade of DR-D2 had no effect on it.

Conclusions

The current data suggests the possible influence of dopaminergic receptors D2 and TFEB genes on the behavioral changes which is modified by having an aggressive father.

中文翻译：

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攻击性雄性大鼠后代 D2 受体和转录因子 EB 表达改变，并伴有抑郁和焦虑样行为

摘要

研究目的：杏仁核和前额叶皮层 (PFC) 中的多巴胺能系统通过多巴胺能受体 (DR)，尤其是 DR-D1 和 DR-D2 影响恐惧和焦虑。转录因子 EB (TFEB) 是自噬-溶酶体途径的上游蛋白质。

材料和方法

在这项研究中，我们评估了行为情绪变化，以及攻击性雄性大鼠后代杏仁核和 PFC 中 DR-D2 和 TFEB 基因的表达。

结果

观察到焦虑和抑郁样行为，但脑室内注射 DR-D2 拮抗剂（舒必利）已显示可有效减少后代的负面行为变化。此外，攻击性雄性大鼠后代的杏仁核和 PFC 中 DR-D2 基因表达增加，而注射舒必利可显着降低其表达。在攻击性雄性大鼠后代的杏仁核和 PFC 中，TFEB 基因表达也降低，但阻断 DR-D2 对其没有影响。

结论

目前的数据表明，多巴胺能受体 D2 和 TFEB 基因可能对行为改变产生影响，而这种改变会因有一个好斗的父亲而改变。