ABSTRACT The aim of this study was to investigate the response of chromosomes in typical human and plant cells under applied low-frequency magnetic fields at low and high intensities. Neuronal-like cells and roots of Allium sativum and Vicia faba were used to investigate chromosome's response to a static and 50 Hz magnetic fields at intensities ranging from 1 mT to 0.8 T, generated by two Helmholtz coils driven by direct current or alternate current voltage. Vertex spectrometer and Olympus microscope with camera were used. A significant decrease in intensity of the phosphate bands in the DNA infrared region was observed by FTIR spectroscopy analysis after exposure of neuronal-like cells to static and 50 Hz magnetic field at low intensity of 1 mT, which can be explained assuming that uncoiling and unpackaging of chromatin constituents occurred after exposure. This effect was directly observed by microscope in roots of Allium sativum and Vicia faba under exposure to a static magnetic field at high intensity of 0.8 T. These findings can be explained assuming that exposure to both low- and high-intensity magnetic fields of chromosomes in typical human and plant cells induces uncoiling and unpackaging of chromatin constituents, followed by chromosome alignment towards the direction of applied magnetic field, providing further demonstration that magnetic fields can induce the orientation of organic macromolecules even at low-intensity values.

中文翻译：

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暴露于低强度和高强度磁场下的典型生物系统中的染色体畸变

摘要 本研究的目的是研究在低强度和高强度低频磁场下典型人类和植物细胞中染色体的响应。神经元样细胞和大蒜和蚕豆的根被用来研究染色体对静态和 50 Hz 磁场的反应，强度范围从 1 mT 到 0.8 T，由直流或交流电压驱动的两个亥姆霍兹线圈产生。使用顶点光谱仪和带摄像头的奥林巴斯显微镜。将神经元样细胞暴露于 1 mT 低强度的静态和 50 Hz 磁场后，通过 FTIR 光谱分析观察到 DNA 红外区域中磷酸盐带的强度显着降低，这可以通过假设染色质成分在暴露后发生解卷和解包来解释。在暴露于 0.8 T 高强度静磁场的情况下，通过显微镜在大蒜和蚕豆的根部中直接观察到了这种效应。这些发现可以通过假设暴露于低强度和高强度磁场中的染色体来解释典型的人类和植物细胞诱导染色质成分的解卷和解包，然后染色体向施加磁场的方向排列，进一步证明即使在低强度值下，磁场也可以诱导有机大分子的取向。