The compound 20(S),25-epoxydammarane-3β,12β,24α-triol (24-hydroxy-panaxadiol or 24-OH-PD), isolated from the red Panax ginseng CA Meyer possesses anticancer activity. Our aim was to study the pharmacokinetic characteristics of 24-OH-PD, which is essential for pre-clinical research during the development of new drugs.

In this study, a simple and sensitive ultra-performance liquid chromatography-mass spectrometry (LC-MS/MS) method was established and used for studying the pharmacokinetics, in vitro protein binding, tissue distribution, and elimination profiles of 24-OH-PD in rats.

24-OH-PD was characterized by linear pharmacokinetics in the dose range of 2.5–10 mg/kg and had relatively longer half-life (4.82–5.45 h) than the other ginsenosides. It had a wide tissue distribution profile in rats and was primarily distributed in the lung. Within 96 h of intravenous administration, 13.84% of 24-OH-PD was excreted out via feces and 0.02% via urine in its unchanged form.

In conclusion, a simple LC-MS/MS method with high sensitivity and selectivity was established for the quantification of 24-OH-PD.

从红色人参CA Meyer分离出的化合物20（S），25-环氧丹玛烷3β，12β，24α-三醇（24-羟基-人参二醇或24-OH-PD）具有抗癌活性。我们的目的是研究24-OH-PD的药代动力学特性，这对于新药开发期间的临床前研究至关重要。

在这项研究中，建立了一种简单而灵敏的超高效液相色谱-质谱（LC-MS / MS）方法，并用于研究24-OH-PD的药代动力学，体外蛋白质结合，组织分布和消除特征在大鼠中。

24-OH-PD的线性药代动力学特征是剂量范围为2.5–10 mg / kg，并且比其他人参皂苷具有相对更长的半衰期（4.82–5.45 h）。它在大鼠中具有广泛的组织分布特征，并且主要分布在肺中。在静脉内给药的96小时内，有13.84％的24-OH-PD通过粪便排泄，而0.02％的尿液以不变的形式排泄。

总之，建立了一种具有高灵敏度和选择性的简单LC-MS / MS方法，用于定量24-OH-PD。