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Hesperidin promotes differentiation of alveolar osteoblasts via Wnt/β-Catenin signaling pathway
Journal of Receptors and Signal Transduction ( IF 2.8 ) Pub Date : 2020-04-20 , DOI: 10.1080/10799893.2020.1752718
Wei Hong 1 , Wenjie Zhang 1
Affiliation  

Abstract Alveolar bone has a high plasticity. The health of alveolar bones directly determines the success of oral implant surgery, and the health and functions of oral and maxillofacial system. Hesperidin (HES) has many pharmacological activities, such as anti-inflammatory, anti-oxidation, promotion of osteoblast differentiation, but its effect on alveolar osteoblasts is rarely reported. Healthy human alveolar osteoblasts were treated by HES (0.1, 1, 10, 100 μmol/l) and Wnt signaling pathway inhibitor Dickkopf-1 (DKK-1). Then the cell osteogenic differentiation was detected by Alizarin Red S staining, and alkaline phosphatase (ALP) activity was detected by ALP kit. Expressions of genetic markers such as runt-related transcription factor 2 (RUNX2), bone morphogenetic protein-2 (BMP2), osterix (OSX), and osteocalcin (OCN) of osteoblasts were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Expressions of Wnt/β-Catenin pathway-related proteins were measured by Western blot (WB) analysis. HES increased numbers of the cells stained red by Alizarin Red S staining, the ALP activity, and the mRNA expression of Runx2, BMP2, OSX, and OCN, and activated the Wnt/β-catenin signaling pathway. However, DKK-1 reversed the differentiation function of human alveolar osteoblasts accelerated by HES. HES promoted the differentiation of human alveolar osteoblasts through the activation of the Wnt/β-Catenin signaling pathway.

中文翻译:

橙皮苷通过Wnt/β-Catenin信号通路促进肺泡成骨细胞分化

摘要 牙槽骨具有很高的可塑性。牙槽骨的健康直接决定了口腔种植手术的成功与否,以及口腔颌面系统的健康和功能。橙皮苷(HES)具有抗炎、抗氧化、促进成骨细胞分化等多种药理活性,但其对肺泡成骨细胞的作用鲜见报道。健康人肺泡成骨细胞用 HES (0.1, 1, 10, 100 μmol/l) 和 Wnt 信号通路抑制剂 Dickkopf-1 (DKK-1) 处理。茜素红S染色检测细胞成骨分化,ALP试剂盒检测碱性磷酸酶(ALP)活性。遗传标记的表达,如矮小相关转录因子 2 (RUNX2)、骨形态发生蛋白-2 (BMP2)、osterix (OSX)、通过定量实时聚合酶链反应 (qRT-PCR) 检测成骨细胞的骨钙素 (OCN) 和骨钙素 (OCN)。Wnt/β-Catenin 通路相关蛋白的表达通过蛋白质印迹 (WB) 分析测量。HES 增加茜素红 S 染色的细胞数量、ALP 活性以及 Runx2、BMP2、OSX 和 OCN 的 mRNA 表达,并激活 Wnt/β-catenin 信号通路。然而,DKK-1 逆转了 HES 加速的人肺泡成骨细胞的分化功能。HES通过激活Wnt/β-Catenin信号通路促进人肺泡成骨细胞分化。HES 增加茜素红 S 染色的细胞数量、ALP 活性以及 Runx2、BMP2、OSX 和 OCN 的 mRNA 表达,并激活 Wnt/β-catenin 信号通路。然而,DKK-1 逆转了 HES 加速的人肺泡成骨细胞的分化功能。HES通过激活Wnt/β-Catenin信号通路促进人肺泡成骨细胞分化。HES 增加茜素红 S 染色的细胞数量、ALP 活性以及 Runx2、BMP2、OSX 和 OCN 的 mRNA 表达,并激活 Wnt/β-catenin 信号通路。然而,DKK-1 逆转了 HES 加速的人肺泡成骨细胞的分化功能。HES通过激活Wnt/β-Catenin信号通路促进人肺泡成骨细胞分化。
更新日期:2020-04-20
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