Abstract Purpose: Hesperidin has anti-inflammatory and anti-oxidant stress effects, but its functions in chronic obstructive pulmonary disease (COPD) remains unknown. This study analyzed the role of hesperidin in COPD mice, aiming to provide a basis for the hesperidin application. Materials and methods: Mice were injected with cigarette smoke extract (CSE) to construct COPD models and then treated with budesonide or hesperidin. Hematoxylin-eosin (HE) and TUNEL assays were used to observe the pathological changes and cell death of lung tissue. The levels of interleukin (IL)-6, IL-8, malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) in bronchoalveolar lavage fluid (BLAF), as well as myeloperoxidase (MPO) content in lung tissues were confirmed. The expression levels of SIRT1, PGC-1α, and p65 proteins were measured by western blotting (WB) analysis. Results: CSE induced inflammatory cell infiltration and cell death in the lung tissues of mice, whereas budesonide and hesperidin effectively alleviated these pathological changes. The levels of IL-6, IL-8, and MDA in BLAF and pulmonary MPO content in the COPD mice were effectively increased, while the levels of SOD and CAT in BLAF were decreased, which could be reversed by budesonide and hesperidin. Moreover, the addition of budesonide or hesperidin reliably accelerated the expression levels of PGC-1α and SIRT1 but suppressed the phosphorylation of p65 in COPD mice. In general, high-dose hesperidin had a stronger regulatory effect on COPD mice. Conclusions: Hesperidin alleviated inflammation and oxidative stress responses in CES-induced COPD mice, associated with SIRT1/PGC-1α/NF-κB signaling axis, which might become a new direction for COPD treatment.

中文翻译：

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橙皮苷减轻COPD小鼠炎症和氧化应激反应的作用可能与SIRT1/PGC-1α/NF-κB信号轴有关

摘要 目的：橙皮苷具有抗炎和抗氧化应激作用，但其在慢性阻塞性肺疾病（COPD）中的作用尚不清楚。本研究分析了橙皮苷在COPD小鼠中的作用，旨在为橙皮苷的应用提供依据。材料与方法：给小鼠注射香烟烟雾提取物（CSE）以构建COPD模型，然后用布地奈德或橙皮苷处理。苏木精-伊红(HE)和TUNEL法用于观察肺组织的病理变化和细胞死亡。支气管肺泡灌洗液（BLAF）中白细胞介素（IL）-6、IL-8、丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）水平，肺组织髓过氧化物酶（MPO）含量为确认的。SIRT1、PGC-1α、和 p65 蛋白通过蛋白质印迹 (WB) 分析测量。结果：CSE诱导小鼠肺组织炎症细胞浸润和细胞死亡，而布地奈德和橙皮苷有效缓解了这些病理变化。COPD小鼠BLAF中IL-6、IL-8、MDA水平和肺MPO含量有效升高，而BLAF中SOD和CAT水平降低，布地奈德和橙皮苷可以逆转这种情况。此外，布地奈德或橙皮苷的添加可靠地加速了 PGC-1α 和 SIRT1 的表达水平，但抑制了 COPD 小鼠中 p65 的磷酸化。总的来说，高剂量橙皮苷对COPD小鼠有更强的调节作用。结论：橙皮苷减轻了 CES 诱导的 COPD 小鼠的炎症和氧化应激反应，