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Expression of pulmonary arterial elastin in rats with hypoxic pulmonary hypertension using H2S
Journal of Receptors and Signal Transduction ( IF 2.8 ) Pub Date : 2020-03-11 , DOI: 10.1080/10799893.2020.1738482
Juan Chen 1 , Haizhou Zhang 2 , Wancheng Yu 2 , Lei Chen 2 , Zhengjun Wang 2 , Tao Zhang 2
Affiliation  

Abstract Object: This study analyses the changes of pulmonary arterial elastin expression inhibited by hydrogen sulfide (H2S) in rats with hypoxic pulmonary hypertension. Method: The research used 30 healthy rats and randomly divided them into control group, hypoxia group, and hypoxia + sodium hydrosulfide group. Each group contains 10 samples. The right catheterization was selected to measure the mean pulmonary artery pressure (mPAP). The RV/LV + S ratio was calculated through separating the right ventricle and the left ventricle plus the interventricular septum. Optical microscopy was used to observe the changes of pulmonary vascular structure. The research used immunohistochemistry to express the levels of elastin and transforming growth factor beta (TGF-β). Results: The ratios of Mpap and RV/LV + S in the hypoxic group exceed the control group. The hypoxia + sodium hydrosulfide group (hypoxia + NaHS) is lower than the hypoxic group. In the hypoxic group, the elastic expressions of medium and small pulmonary artery smooth muscle cells exceed the control group. The expression of elastin in hypoxic + NaHS medium and small pulmonary artery smooth muscle cells is lower than that of the control group.The protein expression levels of α-SM-actin in muscle arterial smooth muscle of pulmonary arterioles in hypoxic group, control group and hypoxic + NaHS group were 49.84% + 6.27%, 56.84% + 6.38%, 23.82% + 3.84%, 27.51% + 3.24%, 29.00% + 4.05%, 34.72% + 3.38%. Conclusion: Hydrogen sulfide in rats with hypoxic pulmonary hypertension can inhibit the expression of elastin in its extracellular matrix, which also has remarkable regulation function in forming HPH and remodeling hypoxic pulmonary vascular structure.

中文翻译:

硫化氢在缺氧性肺动脉高压大鼠肺动脉弹性蛋白的表达

摘要 目的:研究硫化氢(H2S)抑制缺氧性肺动脉高压大鼠肺动脉弹性蛋白表达的变化。方法:本研究选取健康大鼠30只,随机分为对照组、缺氧组和缺氧+硫氢化钠组。每组包含 10 个样本。选择正确的导管插入术测量平均肺动脉压 (mPAP)。RV/LV + S 比值是通过分离右心室和左心室加上室间隔来计算的。光学显微镜观察肺血管结构的变化。该研究使用免疫组织化学来表达弹性蛋白和转化生长因子 β (TGF-β) 的水平。结果:缺氧组Mpap和RV/LV+S比值均超过对照组。缺氧+硫氢化钠组(缺氧+NaHS)低于缺氧组。缺氧组中、小肺动脉平滑肌细胞弹性表达超过对照组。低氧+NaHS中小肺动脉平滑肌细胞弹性蛋白表达低于对照组。 低氧组、对照组和对照组肺小动脉肌动脉平滑肌α-SM-actin蛋白表达水平缺氧+NaHS组分别为49.84%+6.27%、56.84%+6.38%、23.82%+3.84%、27.51%+3.24%、29.00%+4.05%、34.72%+3.38% 结论:硫化氢可抑制缺氧性肺动脉高压大鼠细胞外基质中弹性蛋白的表达,
更新日期:2020-03-11
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