ABSTRACT As an apoplast signal molecule, extracellular ATP (eATP) is involved in the growth regulation of Arabidopsis thaliana seedlings. Recently, RRFT1 was revealed to be involved in eATP- regulated seedling growth. To further verify the role of RRTF1 in seedlings' eATP response, expression of 20 eATP-responsive genes in wild type (Col-0) and RRTF1 null mutant (rrtf1-1) seedlings were investigated by using realtime quantitative PCR. After 0.5 mM ATP stimulation, the response of these genes' expression in rrtf1-1 seedlings was significantly different from that in Col-0 seedlings. Proteins which are encoded by these genes include transcription factors, plasma membrane receptors like kinases, ion influx/efflux transporters and hormone signaling components. The results indicated that RRTF1 may be involved in eATP regulated physiological responses via regulating the expression of some functional genes.

中文翻译：

---

RRFT1（氧化还原反应性转录因子 1）参与拟南芥幼苗中细胞外 ATP 调节的基因表达

摘要 作为质外体信号分子，细胞外 ATP (eATP) 参与拟南芥幼苗的生长调节。最近，发现 RRFT1 参与 eATP 调节的幼苗生长。为了进一步验证 RRTF1 在幼苗 eATP 反应中的作用，通过使用实时定量 PCR 研究了 20 个 eATP 反应基因在野生型 (Col-0) 和 RRTF1 空突变体 (rrtf1-1) 幼苗中的表达。0.5 mM ATP 刺激后，这些基因在 rrtf1-1 幼苗中的表达响应与 Col-0 幼苗中的显着不同。由这些基因编码的蛋白质包括转录因子、质膜受体如激酶、离子流入/流出转运蛋白和激素信号成分。