ABSTRACT Anthocyanins are considered health-promoting phytonutrients; however, anthocyanins strictly occurr in the fruit peel of purple tomato cultivars, making the total anthocyanin content limited per tomato fruit. In this study, we performed a transcriptome analysis between the fruit peel and flesh of a purple tomato cultivar ‘Indigo Rose’ at both the mature green stage and breaking stage. In total, 1,945 differently expressed genes, including 165 transcription factors, were detected between the fruit peel and flesh, both at and after the mature green stage. We further analyzed the transcription of anthocyanin biosynthesis genes and the regulatory genes composing the MYB-bHLH-WD40 (MBW) complex between the fruit peel and flesh at both development stages. In addition, several light-sensing genes and other transcription factor genes, including BBX family genes and WRKY genes, showed different expression patterns between the fruit peel and flesh. These findings deepen our understanding of anthocyanin biosynthesis in tomato fruit peels and facilitate the identification of genes limiting the anthocyanin biosynthesis in tomato fruit flesh.

中文翻译：

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紫色番茄品种‘靛蓝玫瑰’果皮与果肉差异表达基因的比较转录组分析

摘要 花青素被认为是促进健康的植物营养素；然而，紫色番茄品种的果皮中严格存在花青素，使得每个番茄果实的花青素总含量受到限制。在这项研究中，我们对紫色番茄品种“靛蓝玫瑰”在成熟绿色阶段和破碎阶段的果皮和果肉之间进行了转录组分析。在成熟的绿色阶段和之后，在果皮和果肉之间总共检测到 1,945 个不同表达的基因，包括 165 个转录因子。我们进一步分析了花青素生物合成基因的转录和组成 MYB-bHLH-WD40 (MBW) 复合体在两个发育阶段果皮和果肉之间的调控基因。此外，还有几个感光基因和其他转录因子基因，包括 BBX 家族基因和 WRKY 基因，在果皮和果肉之间表现出不同的表达模式。这些发现加深了我们对番茄果皮中花青素生物合成的理解，并有助于鉴定限制番茄果肉中花青素生物合成的基因。