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Antagonistic activity of Pseudomonas aeruginosa from compost against Pythium aphanidermatum and Fusarium solani
Biocontrol Science and Technology ( IF 1.5 ) Pub Date : 2020-04-20 , DOI: 10.1080/09583157.2020.1750562
Hamood M. Al-Ghafri 1 , Rethinasamy Velazhahan 1 , Mohammad S. Shahid 1 , Abdullah M. Al-Sadi 1
Affiliation  

ABSTRACT This study was conducted to investigate the presence of native antagonistic bacteria from compost and to evaluate their ability to suppress Pythium and Fusarium-induced damping-off of cucumber. Seven bacterial strains isolated from compost were screened for their in vitro antagonism against Pythium aphanidermatum and Fusarium solani. Out of these, two strains designated as ISO1 and ISO2 showed significant level of antagonism. Scanning electron microscopic observation of the mycelium of P. aphanidermatum and F. solani at the inhibition zones showed morphological changes in the hyphae including deformation, shrinkage and loss of turgidity. Based on 16S rDNA gene sequence analysis, both bacterial strains (ISO1 and ISO2) were identified as Pseudomonas aeruginosa. The cell-free culture filtrates of P. aeruginosa ISO1 and ISO2 significantly reduced the mycelial growth of P. aphanidermatum and F. solani under in vitro conditions. Compatibility analysis of the bacterial strains using cross-streak assay showed that they are compatible to each other. In addition, both strains were found effective in controlling P. aphanidermatum-induced damping-off of cucumber. Soil application of P. aeruginosa ISO1 and ISO2 reduced the yellowing and stunting of cucumber seedlings caused by F. solani. This study demonstrates the effectiveness of P. aeruginosa strains ISO1 and ISO2 isolated from compost in the management of damping-off of cucumber. It also shows that compost can harbour bacterial strains with antagonistic activity against P. aphanidermatum and F. solani.

中文翻译:

堆肥中铜绿假单胞菌对 Pythium aphanidermatum 和 Fusarium solani 的拮抗作用

摘要 本研究旨在研究堆肥中天然拮抗细菌的存在,并评估它们抑制腐霉和镰刀菌引起的黄瓜猝倒病的能力。筛选了从堆肥中分离出的 7 株细菌菌株,以确定它们对 Pythium aphanidermatum 和 Fusarium solani 的体外拮抗作用。其中,指定为 ISO1 和 ISO2 的两个菌株显示出显着的拮抗水平。对抑菌区的P. aphanidermatum 和F. solani 的菌丝体进行扫描电镜观察,发现菌丝形态发生了变形、收缩和失去稠度的变化。基于16S rDNA基因序列分析,两种菌株(ISO1和ISO2)均被鉴定为铜绿假单胞菌。P的无细胞培养滤液。在体外条件下,铜绿假单胞菌 ISO1 和 ISO2 显着降低了 P. aphanidermatum 和 F. solani 的菌丝体生长。使用交叉条纹测定法对细菌菌株的相容性分析表明它们彼此相容。此外,发现这两种菌株均能有效控制 P. aphanidermatum 诱导的黄瓜猝倒病。土壤中施用铜绿假单胞菌 ISO1 和 ISO2 减少了由 F. solani 引起的黄瓜幼苗黄化和发育迟缓。该研究证明了从堆肥中分离的铜绿假单胞菌菌株 ISO1 和 ISO2 在管理黄瓜猝倒病方面的有效性。它还表明,堆肥可以含有对 P. aphanidermatum 和 F. solani 具有拮抗活性的细菌菌株。使用交叉条纹测定法对细菌菌株的相容性分析表明它们彼此相容。此外,发现这两种菌株均能有效控制 P. aphanidermatum 诱导的黄瓜猝倒病。土壤中施用铜绿假单胞菌 ISO1 和 ISO2 减少了由 F. solani 引起的黄瓜幼苗黄化和发育迟缓。本研究证明了从堆肥中分离的铜绿假单胞菌菌株 ISO1 和 ISO2 在管理黄瓜猝倒病方面的有效性。它还表明,堆肥可以包含对 P. aphanidermatum 和 F. solani 具有拮抗活性的细菌菌株。使用交叉条纹测定法对细菌菌株的相容性分析表明它们彼此相容。此外,发现这两种菌株均能有效控制 P. aphanidermatum 诱导的黄瓜猝倒病。土壤中施用铜绿假单胞菌 ISO1 和 ISO2 减少了由 F. solani 引起的黄瓜幼苗黄化和发育迟缓。该研究证明了从堆肥中分离的铜绿假单胞菌菌株 ISO1 和 ISO2 在管理黄瓜猝倒病方面的有效性。它还表明,堆肥可以含有对 P. aphanidermatum 和 F. solani 具有拮抗活性的细菌菌株。土壤中施用铜绿假单胞菌 ISO1 和 ISO2 减少了由 F. solani 引起的黄瓜幼苗黄化和发育迟缓。该研究证明了从堆肥中分离的铜绿假单胞菌菌株 ISO1 和 ISO2 在管理黄瓜猝倒病方面的有效性。它还表明,堆肥可以含有对 P. aphanidermatum 和 F. solani 具有拮抗活性的细菌菌株。土壤中施用铜绿假单胞菌 ISO1 和 ISO2 减少了由 F. solani 引起的黄瓜幼苗黄化和发育迟缓。该研究证明了从堆肥中分离的铜绿假单胞菌菌株 ISO1 和 ISO2 在管理黄瓜猝倒病方面的有效性。它还表明,堆肥可以含有对 P. aphanidermatum 和 F. solani 具有拮抗活性的细菌菌株。
更新日期:2020-04-20
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