Abstract Bactrocera cucurbitae is a major pest of fruit and its reproductive capacity is greatly affected by high temperature. Utilizing ovarian dissection and transcriptome to examine the reproductive biology, we determined that the reproductive capacity of this species is enhanced after exposure for 1 h to 45 °C. The total number of eggs, daily number of eggs, preoviposition period and egg-hatching rate of flies exposed to the high temperature treatment were 693 eggs, 20.4 eggs/d, 6 d and 63.5% versus 666 eggs, 9.6 eggs/d, 11.7d and 96.3%, respectively, in control held at 25 °C for 1 h. The egg-hatching rate was lower than in control, but their ovaries matured in only 6 d versus 12 d in the control. Transcriptome sequencing revealed that after exposure for 1 h to 45 °C, 37 °C, 25 °C, a sequence of 14,492,121,840 nucleotide and 46,826 uniques were screened, and six target genes were involved in oviposition. As temperatures increased, expression levels of Vg-1, Vg-2 and JH-epoxide hydrolase-2 initially increased and subsequently decreased, but the expression of Vg-3 continued to increase there-after. The Vg-receptor which first decreased but then increased.

中文翻译：

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短期高温条件对葫芦科（Coquillett）（双翅目：实蝇科）产卵及差异基因表达的影响

摘要 葫芦巴实蝇是水果的主要害虫，其繁殖能力受高温影响较大。利用卵巢解剖和转录组检查生殖生物学，我们确定该物种的生殖能力在暴露 1 小时至 45 °C 后增强。暴露于高温处理的苍蝇的卵总数、每日卵数、产卵前期和卵孵化率分别为693个卵、20.4个卵/天、6天和63.5%，而666个卵、9.6个卵/天、11.7个卵。 d 和 96.3%，分别在 25 °C 下保持 1 小时的控制。卵孵化率低于对照组，但卵巢仅在 6 d 内成熟，而对照组则为 12 d。转录组测序显示，暴露于 45°C、37°C​​、25°C 1 h 后，序列为 14,492,121,840 个核苷酸和 46, 筛选了826个独特的基因，6个靶基因参与了产卵。随着温度升高，Vg-1、Vg-2和JH-环氧化物水解酶-2的表达水平先升高后降低，但随后Vg-3的表达继续升高。Vg 受体先减少后增加。