ABSTRACT Electrochemical biosensors are a well-known group of tools used widely in a variety of industries. Due to interactions between analytes and a surface, a measurable signal occurs which can then be processed to quantitative and/or qualitative data. The knowledge of surfaces’ electrochemical properties as well as their pKa is of great importance when it comes to the construction of biosensors based on an electrochemical signal. In our work, we focused on determining electrochemical properties as well as pKa of thiol-based SAMs – commonly used linker layers in biosensors. By applying cyclic voltammetry and electrochemical spectroscopy we were able to describe the overall resistive behaviour of SAMs synthesised with both alkanethiols and thiols with carboxyl and amine groups. Coherent use of contact angle measurements let us also describe the overall polarity of the investigated surfaces. The obtained data of pKa values differed from the ones described for bulk solutions of modifying agents, which yields important information for further modification of the surface. GRAPHICAL ABSTRACT

中文翻译：

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追求构建电化学生物传感器的关键特征——金上自组装单层的电化学和酸碱特性

摘要电化学生物传感器是一组众所周知的工具，广泛用于各种行业。由于分析物和表面之间的相互作用，会产生可测量的信号，然后可以将其处理为定量和/或定性数据。当涉及到基于电化学信号的生物传感器的构建时，表面的电化学特性及其 pKa 的知识非常重要。在我们的工作中，我们专注于确定基于硫醇的 SAM（生物传感器中常用的连接层）的电化学特性和 pKa。通过应用循环伏安法和电化学光谱，我们能够描述由烷硫醇和具有羧基和胺基的硫醇合成的 SAM 的整体电阻行为。接触角测量的连贯使用让我们还可以描述所研究表面的整体极性。获得的 pKa 值数据不同于对改性剂本体溶液所描述的数据，这为表面的进一步改性提供了重要信息。图形概要