Accumulating evidence indicates that lncRNAs can interact with miRNAs to regulate target mRNAs through competitive interactions. However, this mechanism remains largely unexplored in laryngeal squamous cell carcinoma (LSCC). In this study, transcriptome-wide RNA sequencing was performed on 3 pairs of LSCC tissues and adjacent normal tissues to investigate the expression profiles of lncRNAs, miRNAs and mRNAs, with differential expression of 171 lncRNAs, 36 miRNAs and 1709 mRNAs detected. Seven lncRNAs, eight mRNAs and three miRNAs were identified to be dysregulated in patients’ tissues by using qRT-PCR. GO and KEGG pathway enrichment analyses were performed to elucidate the potential functions of these differentially expressed genes in LSCC. Subsequently, a ceRNA (lncRNA-miRNA-mRNA) network including 4631 ceRNA pairs was constructed based on predicted miRNAs shared by lncRNAs and mRNAs. Cis- and transregulatory lncRNAs were analysed by bioinformatics-based methods. Importantly, mRNA-related ceRNA networks (mRCNs) were further obtained based on potential cancer-related coding genes. Coexpression between lncRNAs and downstream mRNAs was used as a criterion for the validation of mRCNs, with the ZNF561-AS1-miR217-WNT5A and SATB1-AS1-miR1299-SAV1/CCNG2/SH3 KBP1/JADE1/HIPK2 ceRNA regulatory interactions determined, followed by experimental validation after siRNA transfection. Moreover, ceRNA activity analysis revealed that different activities of ceRNA modules existing in specific pathological environments may contribute to the tumorigenesis of LSCC. Consistently, both downregulated SATB1-AS1 and ZNF561-AS1 significantly promoted laryngeal cancer cell migration and invasion, indicating their important roles in LSCC via a ceRNA regulatory mechanism. Taken together, the results of this investigation uncovered and systemically characterized a lncRNA-related ceRNA regulatory network that may be valuable for the diagnosis and treatment of LSCC.

越来越多的证据表明，lncRNA可以与miRNA相互作用，通过竞争性相互作用调节靶mRNA。但是，这种机制在喉鳞状细胞癌（LSCC）中仍未开发。在这项研究中，在3对LSCC组织和邻近正常组织上进行了转录组范围的RNA测序，以研究lncRNA，miRNA和mRNA的表达谱，并检测到171个lncRNA，36个miRNA和1709个mRNA的差异表达。通过qRT-PCR，发现在患者组织中有7种lncRNA，8种mRNA和3种miRNA失调。进行了GO和KEGG途径富集分析，以阐明这些差异表达基因在LSCC中的潜在功能。后来，基于lncRNA和mRNA共享的预测miRNA，构建了包括4631个ceRNA对的ceRNA（lncRNA-miRNA-mRNA）网络。通过基于生物信息学的方法分析了顺式和反式调控的lncRNA。重要的是，基于潜在的癌症相关编码基因，进一步获得了mRNA相关的ceRNA网络（mRCN）。使用lncRNA和下游mRNA之间的共表达作为验证mRCN的标准，并确定ZNF561-AS1-miR217-WNT5A和SATB1-AS1-miR1299-SAV1 / CCNG2 / SH3 KBP1 / JADE1 / HIPK2 ceRNA调控相互作用siRNA转染后的实验验证。此外，ceRNA活性分析表明存在于特定病理环境中的ceRNA模块的不同活性可能有助于LSCC的肿瘤发生。一致地，下调的SATB1-AS1和ZNF561-AS1均显着促进喉癌细胞的迁移和侵袭，表明它们通过ceRNA调控机制在LSCC中起重要作用。综上所述，这项研究的结果被发现并系统地表征了与lncRNA相关的ceRNA调控网络，这对于LSCC的诊断和治疗可能是有价值的。