Glucansucrases (GTFs) catalyzes the synthesis of α-glucans from sucrose and oligosaccharides in the presence of an acceptor sugar by transferring glucosyl units to the acceptor molecule with different linkages. The acceptor reactions can be affected by several parameters and this study aimed to determine the optimal reaction parameters for the production of glucansucrase-based oligosaccharides using sucrose and maltose as the donor and acceptor sugars, respectively via a hybrid technique of Response Surface Method (RSM) and Particle Swarm Optimization (PSO). The experimental design was performed using Central Composite Design and the tested parameters were enzyme concentration, acceptor:donor ratio and the reaction period. The optimization studies showed that enzyme concentration was the most effective parameter for the final oligosaccharides yields. The optimal values of the significant parameters determined for enzyme concentration and acceptor:donor ratio were 3.45 U and 0.62, respectively. Even the response surface plots for input parameters verified the PSO results, an experimental validation study was performed for the reverification. The experimental verification results obtained were also consistent with the PSO results. These findings will help our understanding in the role of different parameters for the production of oligosaccharides in the acceptor reactions of GTFs.

葡聚糖蔗糖酶（GTF）通过将葡糖基单元转移到具有不同键的受体分子上，在存在受体糖的情况下催化由蔗糖和寡糖合成α-葡聚糖。受体反应可能受到几个参数的影响，本研究旨在确定使用蔗糖和麦芽糖分别作为供体糖和受体糖的葡糖基蔗糖基寡糖生产的最佳反应参数，这是通过响应表面方法（RSM）的混合技术进行的。和粒子群优化（PSO）。使用中央复合设计进行实验设计，测试参数为酶浓度，受体：供体比和反应时间。优化研究表明，酶浓度是最终寡糖产量的最有效参数。确定的酶浓度和受体：供体比率的重要参数的最佳值分别为3.45 U和0.62。即使输入参数的响应面图也验证了PSO结果，也进行了实验验证研究以进行重新验证。获得的实验验证结果也与PSO结果一致。这些发现将有助于我们理解不同参数在GTF受体反应中寡糖生产中的作用。即使输入参数的响应面图也验证了PSO结果，也进行了实验验证研究以进行重新验证。获得的实验验证结果也与PSO结果一致。这些发现将有助于我们理解不同参数在GTF受体反应中寡糖生产中的作用。即使输入参数的响应面图也验证了PSO结果，也进行了实验验证研究以进行重新验证。获得的实验验证结果也与PSO结果一致。这些发现将有助于我们理解不同参数在GTF受体反应中寡糖生产中的作用。