Abstract

In this study, phytochemical analyses of the chloroform extract of Piper betle L. var. haldia and maghai, Piperaceae, leaves led to the isolation of two new phenolic derivatives: 1-n-decanoyl hydroxy-benzoic acid/1-n-decanoyl phenol (H2) and 3-butylphenol (M1) on the basis of spectroscopic data 1D NMR (¹H, ¹³C) and 2D NMR (¹H - ¹H COSY, HMBC) as well as ESI-MS, FT-IR and HR-ESI-MS analyses. Compounds H2 and M1 showed excellent antioxidant DPPH free radical scavenging activity with IC₅₀ values of 10.66 μ/mL and 13.65 μg/mL compared to ascorbic acid as a standard antioxidant with an IC₅₀ value of 2.52 μg/mL. Evaluation of cytotoxic activity against two human oral cancer cell lines (SCC-40 and SCC-29B) showed significant effect with GI₅₀ values of 24.08 and 33.08 μg/mL for compound H2 and 35.03 and 47.06 μg/mL for compound M1, compared to Doxorubicin® as a standard cytotoxic drug with GI₅₀ value of < 10 μg/mL.

摘要

在本研究中，Piper betle L. var.的氯仿提取物的​​植物化学分析。haldia和maghai，胡椒科，叶子导致基于光谱数据分离出两种新的酚类衍生物：1-正癸酰基羟基苯甲酸/1-正癸酰基苯酚（H2）和3-丁基苯酚（M1） 1D NMR ( ¹ H, ¹³ C) 和 2D NMR ( ¹ H - ¹ H COSY, HMBC) 以及 ESI-MS、FT-IR 和 HR-ESI-MS 分析。化合物H2和M1与作为标准抗氧化剂的抗坏血酸相比，抗坏血酸显示出优异的抗氧化剂 DPPH 自由基清除活性，IC ₅₀值为 10.66  μg /mL 和 13.65  μg /mL，其 IC ₅₀值为 2.52  μg /mL。针对两种人口腔癌细胞系（SCC-40和SCC-29B）的细胞毒性活性的评价显示与GI显著效果₅₀的24.08和33.08的值 μ克/ mL的化合物H2和35.03和47.06  μ克/ mL的化合物M1，与作为标准细胞毒性药物的多柔比星相比，GI ₅₀值 < 10  μ g/mL。