Abstract

Two new ergosterol derivatives namely, chlamydosterols A [(22E,24R)-ergosta-7,22-diene-3β,5α,6β-triol 6-decanoate] (1) and B [5α,6β,25-trihydroxy-(22E,24R)-ergosta-7,22-dien-3-one] (5) and three known ergosterols: ergosta-7,22-dien-3β-ol (2), ergosta-5,7,22-triene-3β-ol (3), and ergosta-7,22-diene-3β,5α,6β-triol (4) were separated from the EtOAc extract of the endophytic fungus Fusarium chlamydosporum isolated from Anvillea garcinii (Asteraceae) leaves growing in Saudi Arabia. Their structural assignment was accomplished by various spectroscopic analyses, as well as comparing with the published data. The 5-lipoxygenase (5-LOX) inhibitory potential of the isolated metabolites was assessed. Compounds 1 and 3 displayed moderate 5-LOX inhibitory potential with IC₅₀s 3.06 and 3.57 µM, respectively compared to indomethacin (IC₅₀ 1.13 µM).

摘要

两种新的麦角甾醇衍生物，即衣藻甾醇 A [(22 E ,24 R )-ergosta-7,22-diene-3β,5α,6β-triol 6-decanoate] ( 1 ) 和 B [5α,6β,25-trihydroxy- (22 E ,24 R )-ergosta-7,22-dien-3-one] ( 5)和三种已知的麦角甾醇：ergosta-7,22-dien-3β-ol ( 2 )、ergosta-5,7,22 -triene-3β-ol ( 3 ) 和 ergosta-7,22-diene-3 β ,5α,6β -triol ( 4)从Anvillea garcinii分离的内生真菌Fusarium chlamydosporum的 EtOAc 提取物中分离（菊科）叶子生长在沙特阿拉伯。它们的结构分配是通过各种光谱分析以及与已发表的数据进行比较来完成的。评估了分离代谢物的 5-脂氧合酶 (5-LOX) 抑制潜力。与吲哚美辛 (IC ₅₀ 1.13 µM)相比，化合物1和3显示出中等的 5-LOX 抑制潜力，IC ₅₀分别为 3.06 和 3.57 µM 。