Abstract

Citrus fruits are rich sources of bioactive compounds and their consumption is associated to health-promoting effects. Citrus processing wastes contain bioflavonoids and other high added value compounds. The aim of this study was to evaluate the antiallergic properties of a new phytoextract obtained by citrus wastes and peels. Blood orange and lemon processing wastes were used to produce a Red orange and Lemon Extract (RLE). Blood samples from 30 allergic donors were collected and used to evaluate the basophil activation (CD203c) and degranulation (CD63) by stimulation trough allergen with and without the RLE. Reduced basophil expression of CD203c and CD63 were observed in RLE + Allergen treated samples, with −20.21% of CD203c expression (p < 0.0001) and −54.11% of CD63 expression (p < 0.0001), compared to Allergen treated samples. The RLE evidenced a good antiallergic activity, mainly acting on basophils degranulation, and therefore reducing the key event of pro-inflammatory mediators release after allergic stimuli.

摘要

柑橘类水果富含生物活性化合物，食用它们与促进健康有关。柑橘加工废物含有生物类黄酮和其他高附加值化合物。本研究的目的是评估从柑橘废料和果皮中提取的新植物提取物的抗过敏特性。血橙和柠檬加工废料用于生产红橙和柠檬提取物 (RLE)。收集了来自 30 名过敏性供体的血液样本，用于评估嗜碱性粒细胞活化 (CD203c) 和脱颗粒 (CD63)，通过刺激谷过敏原，有和没有 RLE。在 RLE + 过敏原处理的样本中观察到 CD203c 和 CD63 的嗜碱性粒细胞表达降低，CD203c 表达为 -20.21% ( p  < 0.0001)，CD63 表达为 -54.11% ( p < 0.0001)，与过敏原处理的样品相比。RLE 具有良好的抗过敏活性，主要作用于嗜碱性粒细胞脱颗粒，从而减少过敏刺激后促炎介质释放的关键事件。