Abstract

Several factors affect the vegetative growth of fungi, such as temperature, pH, and culture medium. In addition to mycelial growth, these factors affect metabolite production. There are limited studies that have identified the metabolites produced by the fungus Bjerkandera adusta, which have potential biotechnological applications. Here, we evaluated the effects of temperature, culture medium, and incubation time on the production of mycelial mass and metabolites of B. adusta isolated from Pinus taeda. The highest mycelial mass was obtained at 24 °C, in the potato dextrose and malt extract media, upon incubation for 28 and 35 days. The disaccharide α-α-trehalose was for the first time isolated and identified by X-ray diffraction in this fungal genus.

摘要

有几个因素会影响真菌的营养生长，例如温度、pH 值和培养基。除了菌丝生长外，这些因素也会影响代谢物的产生。有限的研究确定了真菌Bjerkandera adusta产生的代谢物，这些代谢物具有潜在的生物技术应用。在这里，我们评估了温度、培养基和孵育时间对从火炬松中分离的B. adusta菌丝体和代谢物产生的影响。在 24 °C 下，在马铃薯葡萄糖和麦芽提取物培养基中培养 28 天和 35 天后，菌丝体质量最高。双糖α-α-海藻糖是首次在该真菌属中通过X射线衍射分离和鉴定。