The widespread use of pesticides is beneficial for food production; however, there are numerous adverse consequences reported in the ecosystem and humans associated with exposure to these contaminants. The pyrethriod bifenthrin (BIF) is utilized for (1) maintenance, growth, and storage of agricultural products; (2) control of internal and external parasites of farm animals; and (3) eradication of insects threatening public health. Numerous data are available regarding environmental and ecological impact of pyrethriods on the central and peripheral nervous systems; however few studies focused on non-target tissues especially in humans. Therefore, the aim of this investigation was to determine the potential cytotoxic effects of BIF on a non-target tissue using human colorectal HCT-116 cells as a model. Data demonstrated that BIF reduced cell viability and disrupted mitochondrial functions which were accompanied by increased reactive oxygen species (ROS) levels indicating the presence of oxidative stress. BIF produced a significant elevation in levels of malondialdehyde (MDA) supporting the role of oxidative stress in pesticide-mediated toxicity. Concomitantly, a fall of mitochondrial transmembrane potential (Δψ), consequently producing perturbation of fluidity as well as excitability of cellular membranes was noted. Our results also indicated that BIF induced a rise in DNA damage as evidenced by the comet assay. An increase in mitogen-activated protein kinases (MAPKs), JNK (N-terminal Kinase), p38, and ERK (extracellular-signal-regulated kinase) suggested an apoptotic effect. Data thus indicated that BIF-induced cytotoxicity in human colorectal HCT-116 cells was associated with oxidative stress, mitochondrial dysfunction, and apoptosis.

农药的广泛使用有益于粮食生产；然而，据报告，生态系统和人类与这些污染物的暴露有许多不良后果。拟除虫菊酯联苯菊酯（BIF）用于（1）农产品的维护，生长和储存；（二）控制牲畜的内，外寄生虫；（3）消灭威胁公众健康的昆虫。可获得大量有关拟除虫菊酯对中枢神经系统和周围神经系统的环境和生态影响的数据；然而，很少有研究专注于非目标组织，尤其是人类。因此，本研究的目的是使用人结肠直肠HCT-116细胞作为模型，确定BIF对非目标组织的潜在细胞毒性作用。数据表明，BIF降低了细胞活力并破坏了线粒体功能，并伴有活性氧（ROS）水平升高，表明存在氧化应激。BIF显着提高了丙二醛（MDA）含量，支持了氧化应激在农药介导的毒性中的作用。随之而来的是，线粒体跨膜电位（Δψ）的下降，从而导致流动性的扰动以及细胞膜的兴奋性。我们的结果还表明，BIF诱导了DNA损伤的增加，如彗星试验所证明的。有丝分裂原激活的蛋白激酶（MAPKs），JNK（N端激酶），p38和ERK（细胞外信号调节激酶）的增加表明具有凋亡作用。