Abstract The presence of Fusarium head blight on barley impacts beer quality and can have a significant financial impact on the brewing industry. In this work we investigate the use of a portable GCMS instrument combined with a simple headspace solid phase microextraction method as a potential method for the in-situ detection of fungal growth in barley. Four volatile organic compounds (ethyl acetate, 1-pentanol, 2-pentanone, and 2-hexanone) were identified as indicators of fungal contamination. Barley samples were inoculated in-lab and tested over five days. A positive correlation between peak areas of the indicator compounds and ergosterol was obtained. The method was also applied to some naturally grown barley samples. Our preliminary results demonstrate the potential of the method as a means to determine fungal contamination in-situ.

中文翻译：

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顶空固相微萃取 GCMS 作为一种潜在的原位方法，可用于早期检测大麦中的镰刀菌枯萎病

摘要 大麦上镰刀菌枯萎病的存在会影响啤酒质量，并对酿造业产生重大的财务影响。在这项工作中，我们研究了使用便携式 GCMS 仪器与简单的顶空固相微萃取方法相结合作为原位检测大麦真菌生长的潜在方法。四种挥发性有机化合物（乙酸乙酯、1-戊醇、2-戊酮和 2-己酮）被确定为真菌污染的指标。大麦样品在实验室中接种并测试了五天。获得指示剂化合物峰面积与麦角甾醇之间的正相关。该方法还应用于一些自然生长的大麦样品。我们的初步结果证明了该方法作为原位确定真菌污染的手段的潜力。