Abstract Bacterial contamination is a long-standing problem in the brewing and distilling industry. Extant approaches in measuring contamination levels, or for measuring specific microbes, is either slow or expensive. A very rapid (< 1 hr) DNA method using PCR coupled with a fluorescent molecular beacon, showing a sensitivity able to detect levels of a common contaminant (L. delbrueckii), well before sensory signals are triggered, is described. The approach is highly discriminatory, able to differentiate among contaminating strains within a genus, and even in the presence of a considerable excess of desirable strains. The approach implies ways to determine entry points for contamination to help ameliorate the problem and could save a considerable amount of time, resources, and income for the manufacturer.

中文翻译：

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使用分子信标和两步直接 DNA 扩增方案快速鉴定发酵腐败微生物

摘要 细菌污染是酿造和蒸馏行业长期存在的问题。测量污染水平或测量特定微生物的现有方法要么缓慢要么昂贵。描述了一种非常快速（< 1 小时）的 DNA 方法，该方法使用 PCR 结合荧光分子信标，显示出能够在触发感觉信号之前检测常见污染物（L. delbrueckii）水平的灵敏度。该方法具有高度歧视性，能够区分一个属内的污染菌株，甚至在存在大量所需菌株的情况下。该方法暗示了确定污染入口点的方法，以帮助改善问题，并可以为制造商节省大量时间、资源和收入。