ABSTRACT

The aim of this study was to obtain micropropagation protocol for fibrous, Cannabidiol (CBD) rich, hemp variety Epsilon 68. To fulfil this goal, nodal and tip cutting protocol was developed. In further step, this protocol was compared with several other methods dependent on plant growth regulators with cytokine activity to obtain 4.2 ± 1.43 SD explants per tip. Plants from isolated lines were acclimatized to field conditions, and the content of cannabinoids was evaluated in the inflorescences. Described micropropagation protocol may be useful in breeding of hemp varieties and germplasm conservation.

中文翻译：

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用于大麻 L 快速再生的改良节点切割和芽尖协议

摘要

本研究的目的是获得纤维、富含大麻二酚 (CBD) 的大麻品种 Epsilon 68 的微繁殖协议。为了实现这一目标，开发了节点和尖端切割协议。在进一步的步骤中，将该协议与其他几种依赖于具有细胞因子活性的植物生长调节剂的方法进行比较，以获得每个尖端 4.2 ± 1.43 SD 外植体。使分离株系的植物适应田间条件，并评估花序中的大麻素含量。所描述的微繁殖方案可用于大麻品种的育种和种质保护。