Abstract A multiple columns and detections liquid chromatography system, including size exclusion chromatography (SEC) and reversed phase liquid chromatography (RPLC), for the analysis of macromolecules and micromolecules in ginseng root and leaf was developed. The columns were connected by two switching valves. Macromolecules were separated on a SEC column (TSK gel SuperMultipore PW-H column, 6 mm× 150 mm, 8 μm) by isocratic elution of 50 mM ammonium acetate aqueous solution, 0.3 mL/min of flow rate and detected by evaporative light scattering detection (ELSD). Micromolecules were analyzed on a Poroshell RP column (Agilent Poroshell 120 SB-Aq column, 4.6 mm × 50 mm, 2.7 µm) with gradient elution of water and acetonitrile, 0.6 mL/min of flow rate and detected by ultraviolet detection (UV). As a result, in the macromolecules chromatogram of ginseng root sample showed two main peaks while only one major peak for ginseng leaf. For micromolecules analysis, 17 compounds (3 nucleosides + 14 saponins) and 17 compounds (3 nucleosides + 1 flavonoid + 13 saponins) were found in ginseng root and leaf, respectively. The developed method is helpful for the quality evaluation of ginseng root and leaf. Graphical Abstract

中文翻译：

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人参根叶多柱检测液相色谱分析

摘要 建立了包括尺寸排阻色谱（SEC）和反相液相色谱（RPLC）在内的多柱检测液相色谱系统，用于分析人参根和叶中的大分子和小分子。色谱柱由两个切换阀连接。大分子在SEC柱（TSK凝胶SuperMultipore PW-H柱，6 mm×150 mm，8 μm）上通过50 mM醋酸铵水溶液等度洗脱，0.3 mL/min流速分离，蒸发光散射检测(ELSD)。在 Poroshell RP 色谱柱（Agilent Poroshell 120 SB-Aq 色谱柱，4.6 mm × 50 mm，2.7 µm）上分析小分子，水和乙腈梯度洗脱，流速 0.6 mL/min，并通过紫外检测器 (UV) 进行检测。因此，在人参根样品的大分子色谱图中，有两个主峰，而人参叶只有一个主峰。对于小分子分析，在人参根和叶中分别发现了 17 种化合物（3 种核苷 + 14 种皂苷）和 17 种化合物（3 种核苷 + 1 种黄酮类化合物 + 13 种皂苷）。建立的方法有助于人参根叶的质量评价。图形概要