Abstract Bilateral band compression (BBC) is a process where a solvent flow perpendicular to the direction of development squeezes bands into a smaller area. BBC of the 10 mm wide lanes of high-performance thin-layer chromatography (HPTLC) separation resulted in more than 6 times increase in peak height and peak area of Canadian goldenrod labdane diterpenes solidagenone and presolidagenone. Simultaneously, the signal-to-noise ratio increased only up to 2.2–2.4 times due to the higher noise level. The BBC increased the relative standard deviations (RSDs) of peak height and area reached three to four times of the original as well. It is known that overpressured layer chromatography (OPLC) separates more efficiently than conventional TLC/HPTLC techniques. In order to increase the bio-detection sensitivity, we combined bioautography, BBC, and fully offline infusion-OPLC using band-shaped sample application/separation. Thyme essential oil has been applied as a multi-component object to show the potential of this process. It is demonstrated that biologically active minor components, which may be missed in case of a conventional process, can also be detected by means of this combined method. GRAPHICAL ABSTRACT

中文翻译：

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层析结合双边谱带压缩寻找活性微量组分的初步结果

摘要 双边带压缩 (BBC) 是一种溶剂流与发展方向垂直的过程，将带压缩到更小的区域。高效薄层色谱 (HPTLC) 分离的 10 毫米宽泳道的 BBC 导致加拿大一枝花拉丹二萜二萜雄酮和预固酮的峰高和峰面积增加了 6 倍以上。同时，由于较高的噪声水平，信噪比仅增加了 2.2-2.4 倍。BBC 将峰高和峰面积的相对标准偏差 (RSD) 提高到原来的三到四倍。众所周知，超压层析 (OPLC) 比传统的 TLC/HPTLC 技术更有效地分离。为了提高生物检测的灵敏度，我们结合了生物自显影、BBC、和使用带状样品应用/分离的完全离线输液-OPLC。百里香精油已被用作多组分物体，以显示该过程的潜力。事实证明，在常规过程中可能会遗漏的生物活性微量成分也可以通过这种组合方法进行检测。图形概要